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Hours
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Mon 10:00-11:00
am (Braun 152 was 151)
Wed
10:00-11:00 am (not every Wednesday, see schedule
for room)
Lab:
Church B168 (Phone Number x6094)
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Student
group list
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Please contact
Cai Tong
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Syllabus
(updated 3/5)
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week
1
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Introduction
to Microscopes (Koehler illumination, Confocal microscopy)
01/03:
Administrative details and Introductions (152
Braun)
(Assignment
1)
Training
sessions to be scheduled with Cai Tong and Andres
Download
LSM310 manual
Download
LSM410 manual
Download
LSM410 full manual
Manuals
for BIF Microscopes (LARGE File)
(Lecture
01)
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01/08:
Hands on with the microscopes (B168 Church) |
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week
2
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Applying
Geometrical Optics (Making the Rochester Cloak)
01/08: Lecture
on Geometrical optics and Rochester Cloak (152 Braun)
(Assignment
2)
(Lecture
02)
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01/10: Building
invisibility cloak with lenses (B168 Church)
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week
3
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3D
Laser Scanning Confocal Microscopy (LSCM)
01/15:
No class (Martin Luther King Day) Institute Holiday
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01/17: Discuss
assignments (152 Braun, 10:00 am)
(Assignment
3)
(Exciter
power meter manual)
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week
4
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Begin
Building a Light Sheet Microscope (openspim.org)
01/22:
Light sheet fluorescence microscopy; Guest lecture by Dr.
Alon Greenbaum (152 Braun) (Lecture
03)
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01/24: Work on
assignments (B168 Church)
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week
5
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Fluorescent Correlation
Spectroscopy (FCS) vs FRAP
01/29:
FCS
vs FRAP Lecture (152 Braun)
(Lecture
04) (Assignment
4) (FCS
guide) (FRAP
guide)
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Afternoon
lab meet in BIF (B133 Beckman Institute) |
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01/31: Final
Project Proposal due!
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week
6
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Live
Imaging Zebrafish embryos
02/05:
Introduction to Zebrafish (152 Braun)
(Lecture
05) (Assignment
5)
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02/07: Student
presentations for Assignment 3 (152 Braun; 10:00 am)
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week
7
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Multispectral
Imaging Lab
02/12:
Multispectral
Imaging; Guest Lecture by Steven Wilbert (152 Braun) (Lecture
06)
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02/14: Meet in BIF
(B133 Beckman Institute)
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week
8
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Work
on Assignments and building light sheet
02/19:
No class
(President's Day) Institute Holiday
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02/21: Work on light
sheet (B168 Church)
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week
9
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Work
on Assignments and building light sheet
02/26:
Student
presentations (10:00 AM, 152 Braun);
Afternoon,
Work on light sheet (B168 Church)
(Light
sheet instructions)
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02/28: Work
on light sheet (B168 Church)
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week
10
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Super-resolution
Microscopy
03/05:
Super-resolution microscopy lecture (152 Braun)
(Lecture
07)
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03/07: Oral
presentations for final project (10 am)
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03/16:
Final Project Due |
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Grading
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50% Lab
assignments
50% Final
project
Download
Final Project Instructions
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Office
Hours
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Andres Collazo,
acollazo AT Caltech.edu
Phone: x2761 or x2863
Friday 2-4 PM, 226 or B133 in the Beckman Institute
Cai
Tong Ng, cng AT Caltech.edu
By
appointment as well.
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Obtaining
keys to Church 68
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Please contact
Cai Tong
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Microscope
Calendar Access
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Students need
to access the calendar system for microscope reservations:
- go to: http://calendar.bic.caltech.edu/.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without
reserving it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete
your reservation on calendar.
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Try to avoid imaging late at night, since no one will be
around to help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris (Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy from the
Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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