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Hours
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Mon 10:00-11:00
am (Braun 151)
Wed
10:00-11:00 am (see schedule for room)
Lab:
Church 68 (Phone Number x6094)
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Student
group list
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Please contact
Yon
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Syllabus
(updated 12/30)
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week
1
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Introduction
to Microscopes (Koehler illumination, Confocal microscopy)
01/04:
Administrative details and Introductions (151
Braun)
(Assignment
1)
Training
sessions to be scheduled with Yon and Andres
Download
LSM310 manual
Download
LSM410 manual
Download
LSM410 full manual
Manuals
for BIF Microscopes
(Lecture
01)
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01/06:
Hands on with the microscopes (68 Church) |
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week
2
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Applying
Geometrical Optics (Making the Rochester Cloak)
01/11: Lecture
on Geometrical optics and Rochester Cloak (151 Braun)
(Assignment
2)
(Lecture
02)
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01/13: Building
invisibility cloak with lenses (68 Church)
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week
3
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2D
Laser Scanning Confocal Microscopy (LSCM)
01/18:
No class (Martin Luther King Day) Institute Holiday
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01/20: Discuss
assignments (68 Church)
(Assignment
3)
(Exciter
manual)
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week
4
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3D LSCM;
Begin Building a Light Sheet Microscope (openspim.org)
01/25:
Light sheet fluorescence microscopy lecture by Dr. Antti
Lignell (151 Braun) (Lecture 03)
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01/27: Work on
assignments (68 Church)
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week
5
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Live
Imaging I: Drosophila embryos
02/01:
Introduction to Drosophila (Yonil Jung, guest lecture) (151
Braun)
(Lecture 04) (Assignment
4)
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02/03: Final
Project Proposal due!
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week
6
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Live
Imaging II: Zebrafish embryos
02/08:
Introduction to Zebrafish (151 Braun)
(Lecture
05) (Assignment
5)
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02/10: Student
presentations for Assignment 3 (60 Church)
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week
7
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Multispectral
Imaging Lab
02/15:
No class (President?s Day) Institute Holiday
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02/17: Meet in BIF
(B133 Beckman Institute)
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week
8
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Fluorescent
Correlation Spectroscopy (FCS)
02/22:
FCS
Lecture (151 Braun)
(Lecture
06)
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02/24: Meet in BIF
(B133 Beckman Institute)
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02/25: Eric Betzig talk
on Super Resolution Microscopy
Physics Research Conference at 4:00 P.M. in 201 E. Bridge
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week
9
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Comparisons
of Different Confocals & Light Sheet
02/29:
Work
on project and this week?s topic
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03/02: Discussion on
this week's topic
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week
10
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Multispectral
Imaging Lecture
03/07:
Multispectral Imaging lecture (151 Braun)
(Lecture
07)
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03/09: Work on project
and this week?s topic
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03/16:
Final Project Due |
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Grading
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50% Lab
assignments
50% Final
project
Download
Final Project Instructions
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Office
Hours
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Andres Collazo,
acollazo AT Caltech.edu
Phone: x2761 or x2863
Friday 2-4 PM, 226 or B133 in the Beckman Institute
Yonil
Jung, jung830307 AT gmail.com
By
appointment as well.
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Obtaining
keys to Church 68
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Please contact
Yon
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Microscope
Calendar Access
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Students need
to access the calendar system for microscope reservations:
- go to: http://calendar.bic.caltech.edu/.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without
reserving it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete
your reservation on calendar.
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Try to avoid imaging late at night, since no one will be
around to help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris (Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy from the
Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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