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Hours
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Mon 10:00-11:00
am (Braun 152 was 151)
Wed
10:00-11:00 am (not every Wednesday, see schedule
for room)
Lab:
Church B168 (Phone Number x6094)
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Student
group list
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Please contact
Steven Wilbert
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Syllabus
(updated 2/18)
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week
1
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Introduction
to Microscopes (Koehler illumination, Confocal microscopy)
01/06:
Administrative details and Introductions (152
Braun)
(Assignment
1)
Training
sessions to be scheduled with Steven and Andres
Download
LSM310 manual
Download
LSM410 manual
Download
LSM410 full manual
Manuals
for BIF Microscopes (LARGE File)
(Lecture
01)
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01/08: Fiji
Intro by Steven Wilbert; bring your laptops (10 am, 101
Kerckhoff)
Lab Time:
Hands on with microscopes (B168 Church) |
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week
2
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3D
Laser Scanning Confocal Microscopy (LSCM)
01/13: Lecture
on Confocal Microscopes (152 Braun)
(Assignment
2) (Lecture
02)
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01/15: Students
present Assignment 1 (10 am, 101 Kerckhoff)
Lab
Time: Collect 3D confocal data sets (B168 Church)
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week
3
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Begin
Building a Light Sheet Microscope (openspim.org)
01/20:
No class (Martin Luther King Day) Institute Holiday
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01/22: Light sheet
fluorescence microscopy; Guest lecture by Dr. Thai Truong
(10 am, 101 Kerckhoff) (Lecture 03)
Lab
Time: Begin building light sheet microscope (B168 Church)
(Assignment
3) (OpenSPIM
paper)
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week
4
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Live
Imaging Zebrafish Embryos
01/27:
Introduction to zebrafish lecture (152 Braun)
(Lecture
04)
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01/29: Students
present Assignment 2 (10 am, 101
Kerckhoff)
Lab
Time: Set up zebrafish embryo timelapse; Demo Zeiss Z1 light
sheet setup (B133 Beckman Institute)
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week
5
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Fluorescent Correlation
Spectroscopy (FCS) vs FRAP
02/03:
FCS
vs FRAP Lecture (152 Braun)
(Lecture
05) (Assignment
4) (FCS
guide) (FRAP
guide)
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02/05: Lab
Time: Collect FCS and FRAP data sets. Afternoon lab meet
in BIF (B133 Beckman Institute)
Final
Project Proposal due!
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week
6
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Multispectral
Imaging Lab
02/10:
Multispectral
Imaging; Lecture by Steven Wilbert (152 Braun)
(Lecture
06)
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02/12: Lab
Time: Spectral imaging of microbial biofilms
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week
7
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Super-resolution
Microscopy
02/17:
No
class (President's Day) Institute Holiday
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02/19:
Super-resolution microscopy lecture (10 am, 101
Kerckhoff)
(Lecture
07) (Assignment
5)
Lab
Time: Compare Airyscan to deconvolution (B133 Beckman
Institute)
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week
8
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Work
on Assignments and building light sheet
02/24:
Fiji - Basic
Image Analysis Lecture by Steven (152 Braun) (Lecture
08)
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02/26: Students
present Assignment 4 (10
am, 101 Kerckhoff)
Lab
Time: Work on assignments and building light sheet
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week
9
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Work
on Assignments and building light sheet
03/02:
No
lecture. Afternoon,
Work on light sheet
(B168
Church)
(Light
sheet instructions)
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03/05: Students
present Assignment 5 (10 am, 101
Kerckhoff)
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week
10
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Student
Presentations of final project
03/09:
Oral
presentations for final project (152 Braun)
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03/11: Oral
presentations for final project (10 am, 101
Kerckhoff); Assignment 3 due.
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03/16:
Final Project Due |
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Grading
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50% Lab
assignments
50% Final
project
Download
Final Project Instructions
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Office
Hours
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Andres Collazo,
acollazo AT Caltech.edu
Phone: x2761 or x2863
Friday 2-4 PM, 226 or B133 in the Beckman Institute
Steven
Wilbert, swilbert AT Caltech.edu
By
appointment as well.
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Obtaining
code for key to B168 Church
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Please contact
Steven Wilbert
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Microscope
Calendar Access
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Students need
to access the calendar system for microscope reservations:
- go to: bioimaging.caltech.edu/.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without
reserving it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete
your reservation on calendar.
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Try to avoid imaging late at night, since no one will be
around to help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris (Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy from the
Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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