* BACTERIAL EXPRESSION
PI-PLC purification
Inclusion body preparation
Ben's E.coli expression and renaturing
(also see Ben's website)
ProteomTech Inc.

* BACULOVIRUS
Baculovirus-transfection
Sf9 Spinner Media Recipe

* Biacore
Covalent oriented coupling
Biacore instructions

* CALCULATING KD's
Equilibrium Dialysis
Scatchard Analysis
B27 binding assay

* CELL SORTING
Sorting of FcRn expressing cell

* CLONING
Preparation of electocompetent E. coli
How to label DNA tubes
PJB's cloning hints
Cloning tips by Astrid
Lance's subcloning procedures
Site-directed mutagenesis protocol

* CRYSTALLISATION
Siliconized coverslips

* ELISA
ELISA
Anti FcRn ELISA

* IMMUNOPRECIPITATION
Immunoprecipitations (FcRn)
Immunoprecipitations (FcRn) short
Immunoprecipitations (HFE)

* IMMUNOSTAINING
Immunostaining of cells
FcRn sorting

* PROTEIN ELECTROPHORESIS
TCA precipitation protocol
Silver staining of SDS-PAGE
SDS-PAGE and Western blot
Western blot

* TISSUE CULTURE
Tissue Culture Guidelines
Maintenance of HFE producing cells
Freezing adherent mammalian cells
Harvesting proteins Chinese Hamster Ovary cells
Splitting adherent mammalian cells

* TRANSFECTION
Lipofection Procedure
Ca-P Transfection MDCK
Stable transfection of CHO-cells
PCR genes from cells

*USDA - APHIS REGULATIONS
APHIS regulations letter
U.S. Customs Protein Import

* MISC
Keeping a lab book
Pichia MeOH induction screening
BMMY, BMGY recipes
Metabolic labeling of cells
Acid elution tips
Christina's notes on FcRn constructs
ZipTip
Preparing figures for publication
Molecular movies
Polyclonal Antiserum Production

*YUMMIES
Jenny's Beef Protocol
Beth's Banana Bread