* MAMMALIAN CELLS IN USE AT PEC
Michael's group meeting presentation
293 cell transfection
* BACTERIAL
EXPRESSION
PI-PLC
purification
Inclusion body
preparation
Ben's
E.coli expression and renaturing
AP205 phage (VLP) expression
* BACULOVIRUS
Baculovirus-transfection
Sf9
Spinner Media Recipe
* Biacore
Covalent oriented coupling
Biacore instructions
SPR protocols
* CALCULATING
KDs
Equilibrium
Dialysis
Scatchard
Analysis
B27
binding assay
* CLONING
Pouring LB-Agar+Antibiotic Plates
Gibson Cloning
Site-Directed Mutagenesis
Restriction Cloning
Preparation
of electocompetent E. coli
How
to label DNA tubes
PJB's
cloning hints
Cloning tips by Astrid
Lance's
subcloning procedures
Site-directed
mutagenesis protocol
bacterial recombination cloning protocol
competent cell prep (6/2012)
tranformation (6/2012)
Z-competent Cell Transformation
2-stage site-directed mutagenesis
* CRYSTALLISATION
Siliconized
coverslips
* ELISA
ELISA
Anti
FcRn ELISA
Polyreactivity assay
Dot Blot ELISA
* IMMUNOPRECIPITATION
Immunoprecipitations
(FcRn)
Immunoprecipitations
(FcRn) short
Immunoprecipitations
(HFE)
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* IMMUNOSTAINING/CELL SORTING
Immunostaining
of cells
FcRn sorting
* SPINNING DISK
Laser calibration
* PROTEIN
ELECTROPHORESIS
TCA precipitation protocol
Silver staining of
SDS-PAGE
SDS-PAGE and Western blot
Western blot
*
PROTEIN PURIFICATION
How to use the AKTA
1G3 Column-short
1G3 Column for FcRn
mutants
Anti-FcRn mAb
Antibody by
Protein G
HFE - short protocol
HFE - full protocol
TFR (his-tagged)
B27
FcRn
Antibodies
kd/cb2m
kd/mb2m
T cell receptor
UL18 from
insect-cell
media
Purification of
His-tagged
proteins
Purification of StrepII-tagged proteins
nickel vs. cobalt
columns
Fca Purification Protocol
Concentrator instructions
Recharging Ni beads
Osmotic
Shock and TRX-htt
purification
Fab production and purification
PSMA purification
anti-FLAG purification
* SPR
Josh Klein SPR slides
SPR techniques (Anton van der Merwe)
* TISSUE
CULTURE
Tissue Culture Guidelines
Maintenance of HFE producing
cells
Freezing adherent mammalian cells
Harvesting proteins Chinese Hamster Ovary cells
Splitting adherent mammalian cells
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* TRANSFECTION
Maria's Maxiprep
Blaise’s Finishing Maxipreps without Finalizers
Lipofection Procedure
Ca-P Transfection MDCK
Stable
transfection
of CHO-cells
PCR genes from cells
*USDA -
APHIS REGULATIONS
APHIS
regulations letter
U.S. Customs
Protein Import
*THERMOFLUOR ASSAY
Thermofluor Assay protocol
* MISC
Keeping a lab book
Pichia MeOH
induction screening
BMMY,
BMGY recipes
Metabolic
labeling of cells
Acid
elution tips
Christina's
notes on FcRn constructs
ZipTip
Preparing
figures for publication
Molecular
movies
Polyclonal Antiserum
Production
* WRITING A PAPER
Paper/proposal writing guidelines
Guidelines for writing clearly
Show dots in plots
*YUMMIES
Jenny's
Beef Protocol
Beth's
Banana Bread
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