Effects of PU.1 on different subsets of fetal thymocytes at 20 hr after introduction

Gene expression measured in three biological replicates of "pro-T" (Thy-1+ CD25+) and "pre-T" (Thy-1+ CD25-) cells and two biological replicates of "primitive" (Thy-1 low) cells

Gene expression relative to control pro-T cells (empty vector Lz, Thy-1+ CD25+), log(10) transformed

+/-PU.1

 Lz Lz PU.1 PU.1 Lz Lz Lz PU.1 PU.1 PU.1 Lz Lz Lz PU.1 PU.1 PU.1

Stage

 prmtv prmtv prmtv prmtv proT proT proT proT proT proT preT preT preT preT preT preT
Ikaros -1.14 -0.28 -0.36 -0.99 0 0 0 -0.6 -0.7 -0.67 -0.02 -0.1 -0.13 -0.25 -0.72 -0.57
Aiolos -1.04 -0.16 -0.1 -0.39 0 0 0 0.16 0.08 0.28 -0.13 0.05 0.45 0.26 0.65 0.78
Pou6f1 -1.21 -0.43 0.58 -0.46 0 0 0 0.81 0.13 0.33 0.09 -0.35 0.13 -0.26 0.49 0.56
Lef.1 0.58 -1.03 0.27 -1.46 0 0 0 0.66 -0.79 -0.55 0.74 -0.55 -0.06 0.63 -0.33 -0.09
GATA.3 -0.95 -0.51 -0.49 -0.99 0 0 0 -0.47 -0.81 0.46 -0.16 -0.44 0.78 -0.53 -0.35 0.52
Rag1 -1.52 -0.5 -1.39 -2.15 0 0 0 -0.73 -0.83 -0.88 -0.21 -0.32 -0.05 -0.51 -0.75 -0.65
CD3e -0.87 -0.47 -0.84 -1.09 0 0 0 -0.38 -0.38 -0.55 -0.15 -0.25 -0.31 -0.19 -0.36 -0.57
pTalpha -2.27 -0.81 -0.84 -1.35 0 0 0 -0.02 -0.08 -0.06 -0.13 -0.3 -0.01 -0.05 -0.45 -0.09
Deltex1 -0.27 -0.51 -0.12 -0.74 0 0 0 0.22 -0.36 0.09 0.6 -0.03 0.17 0.21 0.04 0.67
Notch1 -0.24 -0.26 -0.65 -1.26 0 0 0 0.06 -0.48 -0.08 -0.58 -0.66 -0.41 -0.28 -0.54 -0.19
Notch3 -0.7 -0.32 -0.76 -1.56 0 0 0 -0.16 -0.33 -0.17 -1.04 -0.51 -0.39 -0.45 -0.24 -0.64
TCF.1 -0.37 -0.5 -1.09 -1.54 0 0 0 -0.78 -1.04 -0.7 -0.2 -0.31 -0.05 -0.95 -0.91 -0.57
SpiB -0.22 -0.25 -1.05 -1.22 0 0 0 -0.52 -0.65 -0.43 -0.5 -0.43 -0.3 -1.13 -1.01 -0.67
HEBCan -0.55 -0.39 -0.83 -1.14 0 0 0 -0.46 -0.69 -0.37 -0.28 -0.18 -0.07 -1.7 -0.43 -1.57
E2A 0.21 -0.28 -0.16 -0.69 0 0 0 0.01 -0.41 -0.22 0.04 -0.27 -0.09 -0.29 -0.2 -0.23
c.Myb -0.13 -0.33 -0.94 -1.44 0 0 0 -0.45 -0.96 -0.64 -0.48 -0.57 -0.21 -0.7 -1.02 -0.6
IL7Ra -0.2 -0.19 -0.04 -0.67 0 0 0 -0.32 -0.51 -0.14 -0.04 -0.32 -0.13 -0.54 -0.31 -0.1
Runx1 -0.27 -0.46 -0.19 -0.52 0 0 0 0.07 -0.25 0.13 -0.3 -0.61 -0.14 -0.32 -0.23 -0.01
Runx3 0.35 -0.03 -0.11 -0.37 0 0 0 -0.19 -0.5 -0.35 -0.24 -0.38 -0.31 -0.63 -0.55 -0.59
Mac.1 1.73 0.3 1.98 0.55 0 0 0 1.69 0.32 0.19 -0.52 -1.37 -0.38 1.36 0.01 0.36
Id2 0.57 0.1 0.74 0.26 0 0 0 0.37 -0.04 0.21 -0.29 -0.39 0.03 -0.07 0 -0.03
PU.1.coding 0.73 0.35 2.3 2.04 0 0 0 2.21 2.2 2.24 -0.93 -1.21 -0.21 2.48 2.02 2.26
HEBAlt -0.05 -0.73 -1.11 -0.65 0 0 0 -0.7 -0.25 -1.2 -0.64 -0.16 -0.42 -1.31 -0.28 -0.4
GATA.2 1.18 -2.57 -0.63 -3.12 0 0 0 -1.22 -2.67 -0.33 -0.63 -3.07 0.24 -0.82 -3 -0.16

All data from C.B. Franco, D. D. Scripture-Adams, I. Proekt, T. Taghon, A. H. Weiss, M. A. Yui, S. L. Adams, R. A. Diamond, and E. V. Rothenberg, Proc Natl Acad Sci USA 2006; 103:11993-11998

Gene expression levels in fetal thymus subset controls

Initial effects of PU.1 on gene expression in fetal thymus subsets