Efects of forced PU.1 expression in fetal thymocytes in the presence or absence of Notch signals

Gene expression measured by quantitative real-time RT-PCR
+/-Notch Ligand DL DL DL DL DL DL CTRL CTRL CTRL CTRL CTRL CTRL
+/-PU.1 LZ LZ LZ PU.1 PU.1 PU.1 LZ LZ LZ PU.1 PU.1 PU.1
PU.1.coding 0 0 0 1.98 1.86 1.74 0.08 0.1 -0.12 1.78 1.92 1.81
Deltex.1 0 0 0 -0.11 -0.38 -0.16 -2.25 -2.66 -2.21 -2.93 -2.35 -2.3
HES.1 0 0 0 0.05 -0.14 -0.32 -0.97 -0.67 -0.54 -1.72 -1.23 -1.22
pTalpha 0 0 0 0.07 -0.14 -0.12 -1.3 -1.39 -1.2 -1.39 -1.53 -1.18
Rag.1 0 0 0 -0.49 -0.33 -0.33 -0.05 -0.09 -0.12 -1.3 -0.81 -0.8
CD3g 0 0 0 -0.2 -0.21 -0.29 -0.01 -0.04 -0.05 -0.32 -0.47 -0.38
CD3e 0 0 0 -0.24 -0.18 -0.14 -0.09 0.02 0.06 -0.65 -0.45 -0.39
Zap70 0 0 0 -0.46 -0.41 -0.17 0.01 -0.11 0.28 -1.08 -0.7 -0.36
Lck 0 0 0 -0.28 -0.36 -0.42 -0.26 -0.32 -0.11 -0.84 -0.76 -0.66
LAT 0 0 0 -0.33 -0.36 -0.4 -0.19 -0.24 -0.06 -0.9 -0.84 -0.78
Runx3 0 0 0 -0.1 -0.08 -0.05 0.07 0 0.1 -0.28 -0.12 -0.14
Runx1 0 0 0 0.02 -0.02 0.02 -0.35 -0.53 -0.32 -0.29 -0.33 -0.13
FOG.1 0 0 0 -0.16 -0.35 -0.26 0.01 -0.1 -0.13 -0.85 -0.49 -0.56
GATA.3 0 0 0 0.18 0.14 -0.03 -0.09 -0.35 -0.18 -0.7 -0.61 0.06
TCF.1 0 0 0 -0.41 -0.65 -0.57 -0.12 -0.22 -0.16 -1.14 -0.88 -0.81
Ets2 0 0 0 -0.49 -0.4 -0.56 -0.39 -0.4 -0.38 -1.28 -0.84 -1.02
Ets1 0 0 0 -0.34 -0.24 -0.52 0.02 -0.09 0.04 -1.04 -0.68 -0.66
Ikaros 0 0 0 -0.3 -0.21 -0.02 0.04 -0.13 0.36 -0.52 -0.41 -0.33
Gfi1 0 0 0 -0.27 -0.4 -0.29 -0.26 -0.27 -0.03 -1.31 -0.74 -0.9
c.Myb 0 0 0 -0.11 -0.19 -0.23 -0.23 -0.32 -0.2 -1.05 -0.82 -0.8
HEBCan 0 0 0 -0.15 -0.16 -0.23 0.09 -0.07 -0.01 -0.37 -0.55 -0.39
HEBAlt 0 0 0 -0.89 -0.18 -0.38 -0.97 -1.08 -0.96 -1.56 -1.8 -1.15
E2A 0 0 0 -0.36 -0.29 -0.22 -0.14 -0.43 -0.26 -0.81 -0.6 -0.33
Id1 0 0 0 -0.13 0.2 -0.1 0.19 0.29 0.16 0.13 0.2 0.22
Id2 0 0 0 -0.02 0.14 0.41 0.27 0.22 0.25 0.71 0.85 0.72
Id3 0 0 0 -0.38 -0.24 -0.27 0.23 0.24 0.49 -0.24 0.1 -0.03
Bcl11B 0 0 0 -0.12 -0.26 -0.56 0.01 -0.2 0.18 -0.97 -0.71 -0.69
Bcl11A 0 0 0 0.83 0.07 0.98 0.01 -0.36 0.07 0.77 0.13 0.8
PU.1.endog 0 0 0 0.08 0.02 0.69 -0.15 -0.21 -0.41 -0.02 0.29 0.51
C.EBPa 0 0 0 -0.21 0.63 -0.31 0.5 1.05 0.05 0.3 0.91 0.01
C.EBPb 0 0 0 0.13 0.12 0.08 -0.11 0.01 0.23 -0.09 0.21 -0.07
M.CSF.R.rep 0 0 0 0.81 0.82 0.49 0.2 0.74 -0.77 0.84 0.6 0.5
LysozymeM.repeat 0 0 0 -0.07 0.08 -0.24 0.46 0.39 -0.38 0.47 0.96 -0.17
GM.CSFR.b. 0 0 0 0.3 -0.01 0.33 -0.12 -0.18 -0.1 0.08 0.49 0.35
Mac.1 0 0 0 0.71 0.58 0.78 -0.01 0 0.08 1.21 1.18 1.12
Egr1 0 0 0 -0.39 0.31 0.58 -0.26 0.42 0.28 -0.4 0.39 0.34
Egr2 0 0 0 0.2 0.52 0.56 -0.25 0.14 -0.02 0.16 0.33 0.51
Egr3 0 0 0 -0.23 -0.63 -0.39 -0.68 -1.09 -0.38 -2.44 -2.35 -2.33
Averages of log values
DL DL CTRL CTRL   +/-Notch Ligand
LZ PU.1 LZ PU.1 StdErr +/-PU.1
0 1.86 0.02 1.84 0.055 PU.1.coding
0 -0.22 -2.38 -2.53 0.13 Deltex.1
0 -0.14 -0.73 -1.39 0.117 HES.1
0 -0.07 -1.3 -1.36 0.066 pTalpha
0 -0.39 -0.09 -0.97 0.087 Rag.1
0 -0.24 -0.03 -0.39 0.028 CD3g
0 -0.19 0 -0.5 0.047 CD3e
0 -0.35 0.06 -0.71 0.127 Zap70
0 -0.35 -0.23 -0.75 0.045 Lck
0 -0.36 -0.16 -0.84 0.033 LAT
0 -0.08 0.05 -0.18 0.03 Runx3
0 0.01 -0.4 -0.25 0.045 Runx1
0 -0.26 -0.07 -0.63 0.065 FOG.1
0 0.09 -0.2 -0.42 0.129 GATA.3
0 -0.54 -0.17 -0.94 0.062 TCF.1
0 -0.48 -0.39 -1.04 0.067 Ets2
0 -0.36 -0.01 -0.8 0.076 Ets1
0 -0.18 0.09 -0.42 0.088 Ikaros
0 -0.32 -0.19 -0.98 0.095 Gfi1
0 -0.18 -0.25 -0.89 0.049 c.Myb
0 -0.18 0 -0.43 0.039 HEBCan
0 -0.48 -1 -1.5 0.144 HEBAlt
0 -0.29 -0.28 -0.58 0.083 E2A
0 -0.01 0.21 0.18 0.058 Id1
0 0.18 0.24 0.76 0.066 Id2
0 -0.3 0.32 -0.06 0.068 Id3
0 -0.31 0 -0.79 0.096 Bcl11B
0 0.62 -0.09 0.57 0.19 Bcl11A
0 0.27 -0.26 0.26 0.137 PU.1.endog
0 0.04 0.53 0.41 0.246 C.EBPa
0 0.11 0.04 0.02 0.07 C.EBPb
0 0.71 0.06 0.65 0.233 M.CSF.R.rep
0 -0.08 0.16 0.42 0.216 LysozymeM.repeat
0 0.2 -0.13 0.31 0.082 GM.CSFR.b.
0 0.69 0.02 1.17 0.035 Mac.1
0 0.17 0.15 0.11 0.218 Egr1
0 0.43 -0.04 0.33 0.095 Egr2
0 -0.41 -0.72 -2.38 0.12 Egr3

All effects measured at 40 hr after introduction (~16 hr of infection in absence of stroma + 24 hr in presence of OP9-DL1 cells with or without DL1 expression)
Data for independent biological triplicates expressed relative to control (DL, LZ) in log(10) transformation
All published in: C.B. Franco, D. D. Scripture-Adams, I. Proekt, T. Taghon, A. H. Weiss, M. A. Yui, S. L. Adams, R. A. Diamond, and E. V. Rothenberg, Proc Natl Acad Sci USA 2006; 103:11993-11998