Gene expression measured by quantitative real-time RT-PCR (log10 ratios relative to actin)
Gene |
.DN1 |
.DN1 |
.DN2 |
.DN2 |
DN2A |
DN2A |
.DN2 |
DN3A |
DN3A |
DN3A |
DN3A |
DN3B |
DN3B |
DN3B |
DN3B |
..DP.. |
..DP.. |
DP69- |
DP69- |
.CD8 |
.CD8 |
CD8_69- |
CD8_24- |
.CD4 |
.CD4 |
CD4_69- |
CD4_24- |
.gdT. |
.gdT. |
Bcl11b |
-4.15 |
-4.31 |
-1.37 |
-1.44 |
-1.8 |
-1.74 |
-1.51 |
-0.77 |
-1.17 |
-1.12 |
-1 |
-1.34 |
-1.64 |
-1.52 |
-1.42 |
-1.22 |
-1.37 |
-1.41 |
-1.33 |
-1.89 |
-2.03 |
-1.72 |
-2.18 |
-1.47 |
-1.68 |
-1.42 |
-1.8 |
-1.57 |
-1.85 |
CD3e |
-3.74 |
-3.62 |
-0.29 |
-0.55 |
-1.82 |
-1.92 |
-1 |
0.2 |
0.15 |
0.08 |
0.3 |
-0.17 |
-0.25 |
-0.38 |
-0.12 |
-0.14 |
-0.38 |
-0.49 |
-0.31 |
-0.34 |
-0.35 |
-0.47 |
-0.57 |
-0.25 |
-0.57 |
-0.37 |
-0.28 |
-0.51 |
-0.52 |
Cebpa |
-2.55 |
-1.96 |
-3.82 |
-4.7 |
-3.77 |
-4.57 |
|
-2.47 |
-4.27 |
-3.77 |
|
-3.85 |
-3.38 |
-4.8 |
|
|
-4.66 |
-4.19 |
-3.89 |
|
-3.72 |
-5.74 |
-4.27 |
|
-4.68 |
-5.62 |
-3.8 |
-4.01 |
-4.85 |
Dtx1 |
-2.08 |
-3.12 |
-3.92 |
-2.36 |
-2 |
-1.96 |
-2.21 |
-0.7 |
-1.26 |
-0.96 |
-0.82 |
-1.66 |
-1.68 |
-1.92 |
-1.62 |
-3.01 |
-2.64 |
-2.38 |
-2.39 |
-1.57 |
-1.33 |
-1.46 |
-2.14 |
-1.89 |
-1.96 |
-1.8 |
-1.96 |
-1.96 |
-2 |
Gata3 |
-1.24 |
-1.31 |
-0.62 |
|
-0.7 |
-0.85 |
-0.8 |
-0.47 |
-0.7 |
-0.7 |
-0.37 |
-0.7 |
-0.77 |
-0.72 |
-0.51 |
-0.89 |
-1.04 |
-1.25 |
-1.06 |
-1.68 |
-1.44 |
-1.89 |
-2 |
-0.72 |
-0.96 |
-1.32 |
-1.09 |
-0.41 |
-0.35 |
HEBalt |
-4.15 |
-3.64 |
-0.85 |
-0.96 |
-0.85 |
-1 |
-0.74 |
-0.43 |
-0.7 |
-0.55 |
-0.6 |
-1.06 |
-1.2 |
-0.96 |
-0.85 |
-4.89 |
-3.89 |
-5 |
-3.39 |
-3.77 |
-3.72 |
-5.08 |
-4.27 |
-5.09 |
-5.19 |
-5.62 |
-3.47 |
-2.58 |
-2.54 |
Id2 |
-1 |
-1.37 |
-1.89 |
-2.6 |
-2.08 |
-2.38 |
-2.2 |
-1.11 |
-1.21 |
-1.77 |
-2.62 |
-2.31 |
-2.24 |
-2.41 |
-2.68 |
-3.1 |
-2.82 |
-1.8 |
-1.92 |
-1.8 |
-1.42 |
-1.64 |
-2.03 |
-1.58 |
-1.36 |
-1.72 |
-1.49 |
-1.49 |
-1.51 |
Id3 |
-2.7 |
-2.47 |
-1.52 |
-1.55 |
-2.72 |
-2.72 |
-3.54 |
-1.31 |
-1.3 |
-1.52 |
-1.49 |
-1 |
-1.37 |
-1.09 |
-1.36 |
-1.28 |
-1.23 |
-1 |
-1.06 |
-1.51 |
-1.13 |
-1.17 |
-1.25 |
-1.17 |
-1.39 |
-1.06 |
-1.38 |
-0.92 |
-0.82 |
IL2Rb |
-2.28 |
-2.85 |
-4.89 |
-2.3 |
-4.92 |
-5.11 |
-3.96 |
-4 |
-3.7 |
-4.47 |
-2.05 |
-2.74 |
-2.43 |
-3.64 |
-3.51 |
-3.4 |
|
-1.57 |
-2.12 |
-0.92 |
-0.64 |
-0.96 |
-1.01 |
-1.19 |
-1.29 |
-1.2 |
-1.09 |
-0.96 |
-0.89 |
Lef1 |
-2.03 |
-3.68 |
-1.57 |
-1.7 |
-2.85 |
-2.54 |
-1.46 |
-0.06 |
-0.55 |
-0.29 |
-0.09 |
-0.21 |
-0.34 |
-0.26 |
-0.02 |
-0.28 |
-0.32 |
-0.46 |
-0.48 |
-0.43 |
-0.54 |
-0.85 |
-1 |
0.15 |
-0.28 |
-0.6 |
-0.89 |
-0.29 |
-0.19 |
Notch3 |
-1.96 |
-2.01 |
-0.74 |
-0.8 |
-1.28 |
-1.4 |
-1.33 |
0 |
0.04 |
-0.38 |
-0.28 |
-0.77 |
-0.92 |
-1.28 |
-1.12 |
-1.55 |
-1.48 |
-2.05 |
-1.34 |
-2.32 |
-2.85 |
-2.66 |
-3.7 |
-3.77 |
-3.68 |
-4.36 |
-2.24 |
-1.68 |
-1.92 |
pTa |
-3.28 |
-3.72 |
-1.04 |
-1.31 |
-4.4 |
-4.04 |
-3.05 |
0.34 |
-0.17 |
0.08 |
0.28 |
-0.62 |
-0.7 |
-0.64 |
-0.6 |
-0.8 |
-0.64 |
-2.43 |
-0.96 |
-2.62 |
-3 |
-4.92 |
-4.26 |
-2.02 |
-2.48 |
-4.32 |
-2.57 |
-2.15 |
-2.26 |
PU.1 |
-0.68 |
-0.96 |
-1.54 |
-1.22 |
-0.96 |
-1.04 |
|
-2.49 |
-3.52 |
-1.74 |
|
-3.42 |
-3.3 |
-2.41 |
|
|
-4.62 |
-4.82 |
-2.77 |
|
-3.66 |
-4.19 |
-4.27 |
|
-3.57 |
-4.92 |
-4.23 |
-2.96 |
-3.49 |
Rag1 |
-1.47 |
-2.05 |
-0.44 |
-0.89 |
-1.49 |
-1.54 |
-1.17 |
0.8 |
0.6 |
0.43 |
0.72 |
-0.14 |
-0.33 |
-0.37 |
-0.09 |
0.98 |
0.87 |
-0.72 |
0.8 |
-2.28 |
-0.82 |
-2.92 |
-0.74 |
-2.11 |
-1.72 |
-2.32 |
-0.29 |
-1.85 |
-1.85 |
Scl |
-2.04 |
-1.7 |
-3.8 |
-2.43 |
-1.74 |
-1.74 |
-2.05 |
-4.44 |
-3.62 |
-4.31 |
-4.85 |
-5.08 |
-4.34 |
-4.8 |
-3.72 |
-4.66 |
-4.66 |
-5.66 |
-5.19 |
-5.37 |
-3.72 |
-5.2 |
-4.27 |
-5.11 |
-5.19 |
-5.62 |
-4.23 |
-5.19 |
-5.11 |
Sox13 |
-1.47 |
-1.52 |
-1.09 |
-1.15 |
-1.31 |
-1.66 |
-1.23 |
-1.08 |
-1.06 |
-1.35 |
-0.96 |
-1.22 |
-1.19 |
-1.39 |
-1.08 |
-4.12 |
-3.85 |
-2.58 |
-2.7 |
-4.85 |
-3.57 |
-5.96 |
-4.04 |
-3.57 |
-2.17 |
-3.13 |
-3.05 |
-0.4 |
-0.39 |
SpiB |
-1.25 |
-2.55 |
-1.05 |
-1.4 |
-2.51 |
-3.8 |
-1.92 |
-0.26 |
-0.33 |
-0.51 |
-0.29 |
-1.19 |
-1.2 |
-1.64 |
-1.04 |
-2.36 |
-2.72 |
-2.92 |
-1.96 |
-3.92 |
-3.42 |
-2.21 |
-4.27 |
-4.08 |
-1.7 |
-2.8 |
-2.21 |
-2.62 |
-2.09 |
Tcf7 |
-0.51 |
-0.77 |
0.15 |
0 |
0 |
0 |
0.2 |
0.38 |
0.15 |
0.28 |
0.61 |
0.32 |
0.23 |
0.3 |
0.57 |
0.59 |
0.52 |
-0.17 |
0.28 |
-0.4 |
-0.15 |
-0.66 |
-0.55 |
0.28 |
-0.07 |
-0.18 |
-0.1 |
0.04 |
0.04 |
Zap70 |
-3.05 |
-3.7 |
-1.85 |
-2.02 |
-2.92 |
-2.96 |
-2.12 |
-1.8 |
-1.64 |
-1.77 |
-1.74 |
-1.37 |
-1.35 |
-1.37 |
-1.66 |
-1.19 |
-1 |
-1.33 |
-1.12 |
-1.15 |
-0.82 |
-1.35 |
-0.92 |
-1.26 |
-1.21 |
-1.3 |
-1.15 |
-1.08 |
-1.12 |
Bcl2 |
-1.85 |
-1.6 |
-0.82 |
-0.89 |
-1.22 |
-1.28 |
-0.72 |
-1 |
-1.03 |
-0.96 |
-0.54 |
-1.27 |
-1.35 |
-1.35 |
-0.96 |
-1.74 |
|
-1.23 |
-1.96 |
-0.85 |
-0.7 |
-1.28 |
-1.04 |
-0.89 |
-1.01 |
-1.17 |
-1.36 |
-1.09 |
-1.1 |
CD3g |
-3.1 |
-4.31 |
0.32 |
0.04 |
-1.47 |
-1.02 |
-0.48 |
0.48 |
0.26 |
0.34 |
0.65 |
0.04 |
0.04 |
-0.11 |
0.08 |
-0.21 |
-0.06 |
-0.96 |
-0.09 |
-0.19 |
-0.66 |
-0.85 |
-0.96 |
-2.19 |
-0.43 |
-0.82 |
-0.89 |
-0.42 |
-0.42 |
cKit |
-0.96 |
-0.92 |
-1.09 |
-1.15 |
-0.7 |
-0.7 |
-0.64 |
-1.26 |
-1.34 |
-1.37 |
-1.19 |
-1.77 |
-1.77 |
-1.74 |
-1.57 |
-2.44 |
-3.13 |
-2.54 |
-2.3 |
-2.11 |
-2.68 |
-2.89 |
-2.85 |
-2.35 |
-3 |
-3.41 |
-3.22 |
-1.89 |
-1.89 |
cMyb |
-0.36 |
-0.33 |
0.04 |
0.11 |
0.04 |
-0.09 |
0.11 |
0.18 |
0.15 |
-0.1 |
0.08 |
-0.2 |
-0.19 |
-0.51 |
-0.32 |
-0.09 |
-0.13 |
-0.72 |
-0.14 |
-1.32 |
-2.7 |
-1.82 |
-1.13 |
-1.42 |
-1.6 |
-1.77 |
-1.14 |
-0.58 |
-0.6 |
E2A |
-0.66 |
-1.09 |
-0.7 |
-0.7 |
-0.6 |
-0.52 |
-0.42 |
-0.43 |
-0.6 |
-0.46 |
-0.22 |
-0.62 |
-0.72 |
-0.68 |
-0.46 |
-0.7 |
-0.8 |
-1.34 |
-0.85 |
-1.24 |
-1.33 |
-1.48 |
-1.36 |
-0.92 |
-1.27 |
-1.43 |
-1.24 |
-1.08 |
-1.05 |
Ets1 |
-1.36 |
-1.52 |
-0.77 |
-0.4 |
-0.8 |
-0.89 |
-0.72 |
0.23 |
0 |
-0.19 |
-0.01 |
-0.11 |
-0.21 |
-0.44 |
-0.36 |
0 |
-0.09 |
-0.14 |
-0.15 |
-0.46 |
-0.48 |
-0.44 |
-0.64 |
0 |
-0.24 |
-0.12 |
-0.18 |
-0.2 |
-0.17 |
Ets2 |
-2.8 |
-2.74 |
-2.15 |
-2.37 |
-2.54 |
-2.58 |
-2.49 |
-1.1 |
-1.34 |
-1.62 |
-1.49 |
-1.77 |
-1.77 |
-1.92 |
-1.96 |
-0.8 |
-0.8 |
-1.82 |
-0.92 |
-2.24 |
-2.11 |
-2.44 |
-2.55 |
-1.8 |
-2.03 |
-2.1 |
-1.74 |
-2.04 |
-2.09 |
GAPDH |
0.49 |
0.52 |
0.8 |
0.71 |
0.67 |
0.57 |
0.84 |
0.91 |
0.76 |
0.71 |
1 |
0.9 |
0.9 |
0.85 |
0.96 |
0.58 |
0.54 |
-0.04 |
0.48 |
0.15 |
0.34 |
-0.02 |
-0.08 |
0.45 |
0.15 |
-0.15 |
0.28 |
0.48 |
0.45 |
Gata2 |
-1.47 |
-1.08 |
-3.17 |
-4.66 |
-2.92 |
-3.07 |
|
-3.66 |
-4.27 |
-4 |
|
-4.92 |
-4.64 |
-4.8 |
|
|
-4.66 |
-4.82 |
-5.21 |
|
-3.72 |
-5.74 |
-4.27 |
|
-4.8 |
-5.62 |
-4.23 |
-4.46 |
-4.57 |
Gfi1 |
-1.26 |
-1.42 |
-0.85 |
-0.96 |
-1.12 |
-1.12 |
-1.11 |
-0.32 |
-0.48 |
-0.85 |
-0.64 |
-0.82 |
-0.96 |
-1.18 |
-1.04 |
-0.92 |
-1.03 |
-1.49 |
-0.92 |
-2.19 |
-3.72 |
-2.09 |
-2.26 |
-1.85 |
-1.96 |
-1.85 |
-3.35 |
-1.6 |
-1.55 |
HEBcan |
-0.89 |
-0.89 |
-0.48 |
-0.54 |
-0.62 |
-0.8 |
-0.4 |
-0.2 |
-0.25 |
-0.25 |
-0.02 |
-0.51 |
-0.46 |
-0.43 |
-0.19 |
-0.05 |
-0.13 |
-1.17 |
-0.18 |
-0.82 |
-0.57 |
-1.28 |
-0.58 |
-0.74 |
-0.77 |
-1.23 |
-0.72 |
-0.52 |
-0.55 |
Hes1 |
-0.96 |
-1.13 |
-0.82 |
-0.62 |
-0.92 |
-1.02 |
-0.96 |
-0.19 |
-0.05 |
-0.64 |
-0.41 |
-1.15 |
-0.89 |
-1.44 |
-1.25 |
-3.82 |
-4.41 |
-5.12 |
-2.52 |
-5.37 |
-3.72 |
-5.74 |
-4.27 |
-4.19 |
-3.62 |
-4.92 |
-3.82 |
-2.02 |
-2.2 |
IL2UP (gD) |
-4.15 |
-4.31 |
-3.85 |
-3.92 |
-4.4 |
-4.57 |
-1.57 |
-3.54 |
-3.48 |
-3.96 |
-1.29 |
-5.02 |
-4.64 |
-4.8 |
-1.85 |
-1.7 |
-4.66 |
-5.6 |
-5.21 |
-2.43 |
-3.72 |
-5.74 |
-4.27 |
-1.58 |
-5.19 |
-5.08 |
-3.74 |
-4.74 |
-5.07 |
IL7Ra |
-1.72 |
-2.2 |
-0.89 |
-0.92 |
-0.85 |
-0.96 |
-0.7 |
-0.77 |
-0.92 |
-0.82 |
-0.74 |
-1.1 |
-1.29 |
-1.15 |
-0.89 |
-1.92 |
|
-1.42 |
-1.96 |
-1.01 |
-1.07 |
-1.32 |
-1.48 |
-0.66 |
-0.92 |
-0.96 |
-1.28 |
-1.29 |
-1.14 |
Lat |
-1.54 |
-1.7 |
-0.39 |
-0.49 |
-0.96 |
-1.28 |
-0.96 |
0.04 |
-0.07 |
-0.62 |
-0.43 |
-0.44 |
-0.42 |
-0.77 |
-0.68 |
-0.47 |
-0.47 |
-0.58 |
-0.38 |
-0.89 |
-0.89 |
-0.74 |
-0.96 |
-0.96 |
-0.89 |
-0.48 |
-0.96 |
-0.8 |
-0.92 |
Lck |
-0.89 |
-1.12 |
-0.04 |
-0.25 |
-0.41 |
-0.47 |
-0.27 |
0.54 |
-0.01 |
0.08 |
0.26 |
0.04 |
-0.14 |
-0.24 |
-0.08 |
0 |
-0.04 |
-0.4 |
0 |
-0.58 |
-1.08 |
-0.62 |
-1.19 |
-0.64 |
-0.8 |
-0.58 |
-0.89 |
-0.62 |
-0.66 |
Notch1 |
-0.6 |
-0.7 |
-0.36 |
-0.35 |
-0.41 |
-0.48 |
-0.54 |
0.04 |
-0.08 |
-0.1 |
-0.06 |
-0.44 |
-0.49 |
-0.66 |
-0.52 |
-1.41 |
-0.96 |
-1.58 |
-1 |
-1.32 |
-1.31 |
-1.57 |
-2.02 |
-1.26 |
-1.36 |
-1.57 |
-1.8 |
-1.39 |
-1.27 |
PU1 e1-2 |
-0.92 |
-0.89 |
-1.68 |
-1.42 |
-1.12 |
-1.36 |
-1.23 |
-2.66 |
-3.62 |
-2.03 |
-2.21 |
-3.04 |
-3.58 |
-2.8 |
-2.8 |
-5.06 |
-4.8 |
-5.02 |
-4.23 |
-5.37 |
-2.89 |
-4.77 |
-4.04 |
-5.58 |
-3.72 |
-5.72 |
-4.47 |
-3.09 |
-4.24 |
Runx1 |
0.11 |
-0.32 |
0.11 |
0.11 |
0.08 |
-0.07 |
0.28 |
0.45 |
0.28 |
0.23 |
0.51 |
-0.13 |
-0.18 |
-0.09 |
0.04 |
-0.23 |
-0.35 |
-0.8 |
-0.42 |
-1.19 |
-1.02 |
-1.27 |
-1.21 |
-0.31 |
-0.58 |
-0.74 |
-0.4 |
-0.52 |
-0.54 |
Runx3 |
-0.02 |
-0.32 |
-0.28 |
-0.17 |
-0.21 |
-0.32 |
0 |
-0.54 |
-0.54 |
-0.52 |
-0.12 |
-0.77 |
-0.6 |
-0.7 |
-0.46 |
-0.96 |
-1.24 |
-1.13 |
-1.22 |
-0.58 |
-0.24 |
-0.85 |
-0.64 |
-0.64 |
-0.89 |
-1.24 |
-0.96 |
-0.28 |
-0.32 |
TCR-Ca |
-4.18 |
-4.77 |
-3.96 |
-3.58 |
-4.92 |
-5.11 |
-4.68 |
-3.01 |
-3.17 |
-2.44 |
-2.52 |
-2.42 |
-2.07 |
-2.15 |
-1.96 |
-0.82 |
-0.58 |
-0.92 |
-0.55 |
-0.8 |
-0.72 |
-0.85 |
-0.82 |
-0.44 |
-0.47 |
-0.82 |
-1.09 |
-1.72 |
-1.72 |
|
High Quality Selection of QPCR Data on DN Stages
Gene |
.DN1 |
.DN1 |
DN2A |
DN2A |
DN2B |
DN2B |
DN3A |
DN3A |
DN3A |
Bcl11b
|
-3.85 |
-4.41 |
-1.82 |
-1.8 |
-1.15 |
-1.24 |
-0.96 |
-1.33 |
|
CD3e
|
-3.44 |
-3.27 |
-2.07 |
-1.92 |
-0.49 |
-0.54 |
-0.03 |
-0.2 |
|
Cebpa
|
-1.66 |
-1.55 |
-3.82 |
-4 |
-3.74 |
-3.24 |
-3.03 |
-3.68 |
-3.89 |
Dtx1
|
-2.16 |
-2.72 |
-2.06 |
-2 |
-2.6 |
-2.46 |
-0.82 |
-1.12 |
|
Gata3
|
-1.11 |
-1.27 |
-0.7 |
-0.72 |
-0.42 |
-0.31 |
-0.62 |
-0.66 |
-0.55 |
HEBalt
|
-4.72 |
-4.64 |
-1.05 |
-0.92 |
-0.66 |
-0.62 |
-0.57 |
-0.68 |
|
Id2
|
-1.21 |
-1.42 |
-2.46 |
-2.74 |
-1.82 |
-2.09 |
-1.42 |
-2.38 |
|
Id3
|
-2.26 |
-3.85 |
-2.43 |
-3.52 |
-1.77 |
-1.77 |
-1.55 |
-1.6 |
|
Il2b
|
-2.55 |
-2.66 |
-4.82 |
-3.8 |
-3.68 |
-2.51 |
-4.4 |
-2.82 |
|
Lef
|
-2.21 |
-2.92 |
-2.36 |
-2.44 |
-1.33 |
-1.32 |
-0.28 |
-0.33 |
|
Notch3
|
-2.1 |
-2.01 |
-1.22 |
-1.47 |
-0.92 |
-0.82 |
0.08 |
-0.21 |
|
preTa
|
-4.72 |
-4.64 |
-4.09 |
-4.08 |
-1.42 |
-1.8 |
0 |
-0.17 |
|
PU.1
|
-0.8 |
-0.74 |
-1 |
-1.14 |
-1.66 |
-1.52 |
-2.49 |
-2.25 |
|
Rag1
|
-1.6 |
-1.82 |
-1.44 |
-1.46 |
-0.92 |
-0.96 |
0.58 |
0.3 |
|
Scl
|
-1.85 |
-1.55 |
-1.74 |
-1.74 |
-2.24 |
-2.38 |
-4 |
-5.14 |
|
Sox13
|
-1.48 |
-1.34 |
-1.21 |
-1.39 |
-1.04 |
-1 |
-0.96 |
-1.27 |
|
Spib
|
-1.96 |
-2.28 |
-2.27 |
-2.03 |
-1.6 |
-1.57 |
-0.64 |
-0.7 |
|
Tcf7
|
-0.38 |
-0.57 |
0.15 |
0.11 |
0.4 |
0.4 |
0.26 |
0.3 |
0.32 |
Zap70
|
-4.14 |
-4.01 |
-2.68 |
-2.72 |
-1.92 |
-1.89 |
-1.68 |
-1.54 |
|
Id3-2
|
-3.68 |
-4.64 |
-3.19 |
-4.51 |
-2.07 |
-1.92 |
-1.58 |
-1.85 |
|
|