NORMAL UNPERTURBED IMMATURE THYMOCYTE GENE EXPRESSION

By set 4 5 4 5 4 5 4 5 4 5
Gene DN1.wt DN1.wt DN2.wt DN2.wt DN3a.wt DN3a.wt DN3b.wt DN3b.wt DN4.wt DN4.wt
Aiolos -2.09 -1.88 -3.72 -2.75 -1.98 -1.31 -1.45 -0.57 -0.71 -0.22
CD3e -3.98 -4.25 -2.2 -2.17 -0.62 -0.59 -1.11 -1.15 -1.41 -1.4
Deltex1 -2.27 -2.36 -2.26 -2.24 -0.77 -0.77 -1.2 -1.62 -1.66 -2.87
E2A -0.96 -0.38 -0.81 -0.48 -0.81 -0.66 -0.77 -0.46 -1.08 -0.34
Ets1 -1.11 -0.8 -0.51 -0.42 -0.01 -0.05 -0.14 0.06 -0.4 -0.02
Ets2 -2.65 -2.35 -2.58 -2.55 -1.44 -1.48 -1.8 -1.68 -1.88 -1.62
GATA3 -1.41 -1.33 -0.84 -0.99 -0.72 -0.84 -0.88 -0.74 -0.98 -1.31
"GATA3* (different assay, 1 yr later)" -1.28 -1.26 -0.86 -0.78 -0.69 -0.82 -0.84 -0.54 -1.17 -1.01
Gfi1 -1.19 -1.13 -1.14 -1.13 -0.99 -1.03 -1.21 -1.1 -1.47 -1.14
Gfi1B -1.23 -0.85 -1.39 -1.05 -2.21 -2.06 -2.49 -2.38 -2.82 -2.07
HEB -1.13 -0.69 -0.79 -0.57 -0.24 -0.12 -0.51 -0.1 -0.69 -0.17
Helios -0.94 -0.79 -0.97 -0.48 -1 -0.62 -1.3 -0.57 -1.04 -0.67
Id1 -3.18 -3.77 -2.95 -2.76 -2.46 -2.51 -3.14 -3.57 -2.93 -2.15
Id2 -2.5 -2.15 -2.65 -2.26 -2.28 -2.34 -2.89 -2.73 -3.31 -3.46
Id3 -3.5 -3.16 -2.93 -3.01 -2.2 -2.25 -1.51 -1.75 -1.86 -2.63
Ikaros -0.61 -0.36 -0.85 -0.56 -0.93 -0.53 -1.12 -0.22 -0.72 -0.68
LEF-1 -2.33 -2 -1.71 -1.93 -0.17 0.07 0.33 0.04 0.3 -0.7
MEF (=Elf4) -0.82 -0.4 -0.9 -0.61 -0.65 -0.57 -0.87 -0.6 -1.19 -0.56
Pou6f1 -3.63 -3.51 -3.12 -2.86 -1.63 -1.45 -1.91 -1.58 -1.83 -1.34
PU.1 (=Sfpi1) -0.59 -0.3 -1.08 -0.75 -2.44 -2.03 -3.01 -2.69 -3.14 -3.78
Runx1 -0.27 -0.25 -0.03 -0.09 0.28 0.42 -0.16 -0.17 -0.62 -1.33
Runx2 -0.7 -0.57 -0.94 -0.88 -1.2 -1.08 -2.06 -1.64 -2.89 -2.27
Runx3 -0.37 -0.3 -0.44 -0.51 -0.88 -0.63 -0.96 -1.15 -1.32 -2.01
SpiB-1 -3.14 -3.51 -3.56 -3.3 -1.89 -1.98 -2.68 -3.95 -4.4 -3.35
SpiB-2 -4.07 -4.68 -2.9 -2.84 -1.21 -1.29 -2.33 -2.95 -3.08 -2.6
Tcf7.8 -0.47 -0.06 0.08 0.36 0.35 0.54 0.43 0.75 0.22 0.8
Averages of log transformed values
Gene DN1.avg DN2.avg DN3a.avg DN3b.avg DN4.avg
Aiolos -1.99 -3.23 -1.65 -1.01 -0.47
CD3e -4.12 -2.19 -0.61 -1.13 -1.4
Deltex1 -2.32 -2.25 -0.77 -1.41 -2.26
E2A -0.67 -0.65 -0.74 -0.62 -0.71
Ets1 -0.96 -0.46 -0.03 -0.04 -0.21
Ets2 -2.5 -2.56 -1.46 -1.74 -1.75
GATA3 -1.37 -0.91 -0.78 -0.81 -1.15
"GATA3* (different assay, 1 yr later)" -1.27 -0.82 -0.76 -0.69 -1.09
Gfi1 -1.16 -1.14 -1.01 -1.16 -1.31
Gfi1B -1.04 -1.22 -2.13 -2.43 -2.45
HEB -0.91 -0.68 -0.18 -0.3 -0.43
Helios -0.86 -0.72 -0.81 -0.93 -0.85
Id1 -3.48 -2.85 -2.49 -3.36 -2.54
Id2 -2.32 -2.46 -2.31 -2.81 -3.38
Id3 -3.33 -2.97 -2.23 -1.63 -2.24
Ikaros -0.49 -0.71 -0.73 -0.67 -0.7
LEF-1 -2.17 -1.82 -0.05 0.19 -0.2
MEF (=Elf4) -0.61 -0.75 -0.61 -0.73 -0.88
Pou6f1 -3.57 -2.99 -1.54 -1.75 -1.59
PU.1 (=Sfpi1) -0.44 -0.92 -2.23 -2.85 -3.46
Runx1 -0.26 -0.06 0.35 -0.17 -0.97
Runx2 -0.63 -0.91 -1.14 -1.85 -2.58
Runx3 -0.34 -0.47 -0.76 -1.05 -1.67
SpiB-1 -3.33 -3.43 -1.93 -3.32 -3.87
SpiB-2 -4.38 -2.87 -1.25 -2.64 -2.84
Tcf7.8 -0.26 0.22 0.44 0.59 0.51

"Sets 4&5" adult DN thymocyte subsets, analyzed summer 2005
C57BL/6 mice, 4-6 wk old, sorted based on lack of CD4, 8, TCR, etc. and by c-kit, CD44, CD25, and CD27 expression (DN1-3) and by CD24, CD44, and CD25 expression (DN4)
All published in: E.-S. David-Fung, M. A. Yui, M. Morales, H. Wang, T. Taghon, R. A. Diamond, and E. V. Rothenberg, Immunological Reviews 2006; 209:212-236 . with the exception of Runx2 (not yet published)