Deshaies Lab Publications Ray Deshaies's Graduate and Postdoctoral Publications
Red Text = Reviews Black Text = Research Papers

Deshaies Lab Publications

20. Deshaies, R.J. (1995). Make it or break it: the role of ubiquitin-dependent proteolysis in cellular regulation. Trends Cell Bio. 5, 428-434. Abstract PDF

21. Deshaies, R.J. (1995). The self-destructive personality of a cell cycle in transition. Curr. Opin. Cell Biol. 7, 781-789. Abstract PDF

22. King, R., Peters, J, Deshaies, R.J., and Kirschner, M. (1996). How proteolysis drives the cell cycle. Science 274, 1652-1659. Abstract

23. Verma, R., Chi, Y., and Deshaies, R.J. (1997). Cell-free ubiquitination of cell cycle regulators in budding yeast extracts. Methods Enzymol.283, 366-376.

24. Deshaies, R.J. (1997). Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast. Curr. Opin. Genet. Dev. Biol. 7, 7-16. Abstract

25. Verma, R., Feldman, R. M., and Deshaies, R. J. (1997). SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol. Biol.Cell 8, 1427-1437. Abstract PDF

26. Verma, R., Annan, R., Huddleston, M., Carr, S., Reynard, G., and Deshaies, R.J. (1997). Phosphorylation of Sic1p by G1 cyclin/Cdk is required for its degradation and entry into S phase. Science 278, 455-460. Abstract PDF

27. Feldman, R.M.R., Correll, C.C., Kaplan, K.B., and Deshaies, R.J. (1997). A complex of Cdc4, Skp1, and Cdc53/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1. Cell 91, 221-230. Abstract PDF

28. Henchoz, S., Chi, Y., Catarin, B., Herskowitz, I., Deshaies, R. J., and Peter, M. (1997). Phosphorylation and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev. 11, 3046-3060. Abstract PDF

29. Lyapina, S. L., Correll, C. C., Kipreos, E. T., and Deshaies, R. J. (1998). Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein. Proc. Natl. Acad. Sci. USA 95, 7451-7456. Abstract PDF

30. Peters, J.-M., King, R.W., and Deshaies, R.J. (1998). Cell cycle control by ubiquitin-dependent proteolysis. In Ubiquitin and the Biology of the Cell. ed. J.-M. Peters et al. Plenum Press, NY.

31. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W.S., Jang, J., Shevchenko, A., Charbonneau, H., and Deshaies, R.J. (1999). Exit from mitosis triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97, 233-244. Abstract PDF

32. Straight, A.F., Shou, W., Dowd, G.J., Turck, C.W., Deshaies, R.J., Johnson, A.D., and Moazed, D. (1999). Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell, 97, 245-256. Abstract PDF

33. Seol, J.H., Feldman, R.M.R., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., Chi, Y., Galova, M., Claypool, J., Sandmeyer, S., Nasmyth, K., Shevchenko, A., and Deshaies, R.J. (1999). Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev. 13, 1614-1626. Abstract PDF

34. Freed, E., Lacey, K.R., Huie, P., Lyapina, S.A., Deshaies, R.J., Stearns, T., and Jackson, P.K. (1999). Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13, 2242-2257. Abstract PDF

35. Deshaies, R.J. (1999). SCF and cullin/RING H2-based ubiquitin ligases. Annu. Rev. Cell Dev. Biol. 15, 435-467. Abstract PDF

36. Deshaies, R.J. and Jacks, T. (1999). Cell multiplication. Peering in and peering out: regulation of and by the cell cycle. Curr. Opin. Cell Biol. 11, 705-7. Abstract PDF

37. Verma, R. and Deshaies, R.J. (2000). A proteasome howdunit: the case of the missing signal. Cell 101, 341-344. Abstract PDF

38. Deshaies, R.J. and Meyerowitz, E.M. (2000). COP1 patrols the night beat. Nature Cell Biol. 2, E102-E104. Abstract PDF

39. Reynard, G. J., Reynolds, W., Verma, R., and Deshaies, R. J. (2000). CKS1 is required for G1 cyclin/CDK activity in budding yeast. Mol. Cell Biol. 20, 5858-5864. Abstract PDF

40. Verma, R., Chen, S., Feldman, R., Schieltz, D., Yates, J., Dohmen, J., and Deshaies, R.J. (2000). Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol. Biol. Cell 11, 3425-3439. Abstract PDF

41. Blondel, M., Galan, J.M., Chi, Y., Lafourcade, C., Longaretti, C., Deshaies, R.J., and Peter, M. (2000). Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J. 19, 6085-6097. Abstract PDF

42. Annan, R.S, Huddleston, M.J., Verma, R., Deshaies, R.J., and Carr, S.A. (2001). A multidimensional electrospray MS-based approach to phosphopeptide mapping. Anal. Chem. 73, 393-404. Abstract PDF

43. Lippincott, J., Shannon, K.B., Shou, W., Deshaies, R.J., and Li, R. (2001). The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. Journal of Cell Science 114,1379-86. Abstract PDF

44. Traverso, E.E., Baskerville, C., Liu, Y., Shou, W., James, P., Deshaies, R.J. and Charbonneau, H. (2001). Characterization of the Net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. J Biol. Chem. 276, 21924-31. Abstract PDF

45. Seol, J.H., Shevchenko, A., Shevchenko, A., and Deshaies, R.J. (2001). Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3, 384-91. Abstract PDF

46. Galan, J.M., Wiederkehr, A., Seol, J.H., Haguenauer-Tsapis, R., Deshaies, R.J., Riezman, H., and Peter, M. (2001). Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol. Cell Biol. 9, 3105-17. Abstract PDF

47. Chi, Y., Huddleston, M.J., Zhang, X., Young, R.A., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2001). Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase. Genes Dev. 15,1078-1092. Abstract PDF

48. Schwechheimer, C., Serino, G., Callis J., Crosby, W.L., Lyapina, S., Deshaies, R.J., Gray, W.M., Estelle, M., and Deng, X.W. (2001). Interactions of COP9 Signalosome with the E3 ubiquitin ligase SCFTIR1 in mediating auxin response. Science 292, 1379-1382. Abstract PDF

49. Lyapina, S., Cope, G., Shevchenko, A., Serino, G., Tsuge, T., Zhou, C., Wolf, D.A., Wei, N., Shevchenko, A., and Deshaies, R.J. (2001). Promotion of NEDD8-CUL1 conjugate cleavage by COP9 Signalosome. Science 292, 1382-1385. Abstract PDF

50. Mah, A.S., Jang, J., and Deshaies, R.J. (2001). Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc. Natl. Acad. Sci. USA, 98, 7325-30. Abstract PDF

51. Sakamoto, K.M., Kim, K.B., Kumagai, A., Mercurio, F., Crews, C.M., and Deshaies R.J. (2001). Protacs: chimeric molecules that target proteins to the SCF complex for ubiquitination and degradation. Proc. Natl. Acad. Sci. USA, 98, 8554-9. Abstract PDF

52. Zhou, C., Seibert ,V., Geyer, R., Rhee, E., Lyapina, S., Cope, Greg., Deshaies, R.J., and Wolf, D.A. (2001). The fission yeast COP9/ signalosome is involved in cullin modification by ubiquitin-related Ned8p. BMC Biochemistry, 2, 7. Abstract PDF

53. Shou, W., Sakamoto, K.M., Keener, J., Morimoto, K.W., Traverso, E.E., Azzam, R., Hoppe, G.J., Feldman, R.M.R., DeModena, J., Moazed, D., Charbonneau, H., Nomura, M., and Deshaies, R.J. (2001). Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Mol. Cell, 8, 45-55. Abstract PDF

54. Verma, R., McDonald, W.H., Yates, J.R. III, and Deshaies, R.J. (2001). Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields fully active S phase cyclin-Cdk. Mol. Cell, 8, 439-448. Abstract PDF

55. Wu, G., Lyapina, S., Das, I., Li, J., Guerney, M., Chui, I., Deshaies, R.J. and Kitajewski, J. (2001). SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation. Mol. Cell Biol. 21, 7403-7415. Abstract PDF

56. Deshaies, R.J., and Eilers, M. (2001). Editorial Review: Cell Multiplication. Curr. Opin. Cell Biol. 13, 729-730. PDF

57. Deshaies, R.J., and Ferrell, J.E. (2001). Multisite phosphorylation and the countdown to S phase. Cell 107, 819-822. Abstract PDF

58. Shou, W., and Deshaies, R.J. (2002). Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet 3,4. Abstract PDF

59. Shou, W., Azzam, R., Chen, S.L., Huddleston, M.J., Baskerville, C., Charbonneau, H., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2002). Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol Biol. 3,3. Abstract PDF

60. Shou, W., Verma, R., Annan, R.S., Huddleston, M.J., Chen, S.L., and Deshaies R.J. (2002). Mapping phosphorylation sites in proteins by mass spectrometry. Methods Enzymol. 351, 279-296. PDF

61. Loughrey Chen S., Huddleston, M.J., Shou, W., Deshaies, R.J., Annan, R.S., and Carr, S.A. (2002). Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Mol. Cell. Prot. 1,186-96. Abstract PDF

62. Deshaies, R.J., Seol, J.H., McDonald, W.H., Cope, G., Lyapina, S., Shevchenko, A., Shevchenko, A., Verma, R., and Yates, J.R., 3rd (2002). Charting the protein complexome in yeast by mass spectrometry. Mol. Cell. Prot. 1, 3-10. Abstract PDF

63. Verma, R., Aravind, L., Oania, R., McDonald, W.H., Yates, J.R., III, Koonin, E.V., and Deshaies, R. J. (2002). Role of Rpn11 Metalloprotease in Deubiquitination and Degradation by the 26S Proteasome. Science 298, 611-614. Abstract PDF Suppl

64. Cope, G.A., Suh, G.S.B., Aravind, L., Schwarz, S.E., Zipursky, S.L., Koonin, E.V., and Deshaies, R.J. (2002). Role of Predicted Metalloprotease Motif of Jab1/Csn5 in Cleavage of NEDD8 from CUL1. Science, 298, 608-611. Abstract PDF Suppl

65. Park, C.J., Song, S., Lee, P.R., Shou, W., Deshaies, R.J., and Lee K.S. (2003). Loss of CDC5 function in saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics 163, 21-33. Abstract PDF

66. Petroski, M.D. and Deshaies, R.J. (2003). Context of multiubiquitin chain attachment influences the rate of Sic1 degradation. Mol. Cell 11, 1435-1444. Abstract PDF

67. Petroski, M.D. and Deshaies, R.J. (2003). Redundant degrons ensure the rapid destruction of sic1 at the g1 /s transition of the budding yeast cell cycle. Cell Cycle 2, 410-11. Abstract PDF

68. Cope, G.A. and Deshaies, R.J. (2003). COP9 signalosome: a multifunctional regulator of SCF and other cullin-based ubiquitin ligases. Cell 114, 663-71. Abstract PDF

69. Lipford, J.R. and Deshaies, R.J. (2003). Diverse roles for ubiquitin-dependent proteolysis in transcriptional activation. Nat. Cell Biol. 5, 845-50. Abstract PDF

70. Sakamoto, K.M., Kim, K.B., Verma, R., Ransick, A., Stein, B., Crews, C.M., and Deshaies, R.J. (2003). Development of protacs to target cancer-promoting proteins for ubiquitination and degradation. Mol. Cell Proteomics 2,1350-1358. Abstract PDF

71. Ambroggio, X.I., Rees, D.C., and Deshaies, R.J. (2004). JAMM: A metalloprotease-like zinc site in the proteasome and signalosome. PLoS Biol. 2, 0113-0119. Abstract PDF

72. Graumann G, Dunipace, LA, Seol JH, McDonald, WH, Yates JR III, Wold BJ, and Deshaies, RJ. (2004). Applicability of TAP-MudPIT to pathway proteomics in yeast. Mol. Cell Proteomics 3, 226-237. Abstract PDF

73. Schneekloth, J.S. Jr., Fonseca, F.N., Koldobskiy, M., Mandal, A., Deshaies, R., Sakamoto, K., and Crews, C.M. (2004). Chemical genetic control of protein levels: selective in vivo targeted degradation. J. Am. Chem. Soc. 126:3748-3754. Abstract PDF Suppl

74. Wertz, I.E., O'Rourke, K.M., Zhang, Z., Dornan, D., Arnott, D., Deshaies, R.J., and Dixit, V.M. (2004). Human De-etiolated-1 regulates cJun by assembling a CUL 4A ubiquitin ligase. Science 303:1371-1374. Abstract PDF Suppl

75. Verma, R., Oania, R., Graumann, J., and Deshaies, R.J. (2004). Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system. Cell 118, 99-110. Abstract PDF Suppl Table1 Table2

76. Azzam, R., Chen, S.L., Shou, W., Mah, A.S., Alexandru, G., Nasmyth, K., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2004). Phosphorylation by cyclin B-Cdk underlies release of mitotic exit. Science 305, 516-519. Abstract PDF Suppl

77. Verma, R., Peter, N.R., Tochtrop, G.P., Sakamoto, K.M., D'Onofrio, M., Varadan, R., Fushman, D., Deshaies, R.J., and King, R.W. (2004). Inhibition of proteasome-dependent degradation by a small molecule that binds the ubiquitin chain. Science 306, 117-120. Abstract PDF Suppl

78. Petroski, M.D. and Deshaies, R. J. (2005). Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell Biol. 6, 9-20. Abstract PDF Suppl

79. Mayor, T., Lipford, J.R., Graumann, J., Smith G.T., and Deshaies, R.J. (2005). Analysis of poly-ubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets. Mol. Cell Proteomics 4, 741-751. Abstract PDF

80. Lipford, J.R., Smith, G.T., Chi, Y., and Deshaies, R.J. (2005). A putative stimulatory role for activator turnover in gene expression. Nature 438, 113-116. Abstract PDF Suppl1 Suppl2

81. Mah, A.S., Elia, A.E.H., Devgan, G, Ptacek, J., Schutkowski, M., Snyder, M., Yaffe, M.B., and Deshaies, R.J. (2005). Substrate specificity analysis of protein kinase complex Dbf20Mob1 by peptide library and proteome array screening. BMC Biochemistry 6, 22. Abstract PDF

82. Mayor, T. and Deshaies, R.J. (2005). Two-step affinity purification of multiubiquitylated proteins from Saccharomyces cerevisiae. Methods Enzymol. 399, 385-392. Abstract PDF

83. Verma, R. and Deshaies, R.J. (2005). Assaying degradation and deubiquitination of a ubiquitinated substrate by purified 26S proteasomes. Methods Enzymol. 398, 391-399. Abstract PDF

84. Petroski, M.D. and Deshaies, R.J. (2005). In vitro reconstitution of SCF substrate ubiquitination with purified proteins. Methods Enzymol. 398, 143-158. Abstract PDF

85. Petroski, M.D. and Deshaies, R.J. (2005). Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34. Cell 123, 1107-1120. Abstract PDF

86. Cope, G.A. and Deshaies, R.J. (2006). Targeted silencing of JabI /Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels. BMC Biochemistry 7, 1. Abstract PDF

87. Sharon, M., Taverner, T., Ambroggio, X.I., Deshaies, R.J., and Robinson, C.V. (2006). Structural organization of the 19S proteasome lid: Insights from MS of intact complexes. PloS Biol 4:e267. Abstract PDF

88. Petroski, M.D., Kleiger, G., and Deshaies, R.J. (2006). Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex. Mol Cell 24, 523-534. Abstract PDF Suppl

89. Ghaboosi, N. and Deshaies, R.J. (2007). A conditional yeast E1 mutant blocks the ubiquitin­proteasome pathway and reveals a role for ubiquitin conjugates in targeting Rad23 to the proteasome. Mol. Biol. Cell 18, 1953-1963. Abstract PDF

90. Mayor, T., Graumann, J., Bryan, J., Maccoss, M.M., and Deshaies, R.J. (2007). Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway. Mol. Cell. Proteomics 6, 1885-1895. Abstract PDF Suppl

91. Haririnia, A., Verma, R., Purohit, N., Twarog, M.Z., Deshaies, R.J., Bolon, D., and Fushman, D. (2008). Mutations in the hydrophobic core of ubiquitin differentially affect its recognition by receptor proteins. J. Mol. Biol. 375, 979-996. Abstract PDF

92. Goh, A.M., Walters, J.K., Elsasser, S., Verma, R., Deshaies, R.J., Finley, D., and Howley, P.M. (2008). Components of the ubiquitin-proteasome pathway compete for surfaces on Rad32 family proteins. BMC Biochem. 9, 4. Abstract PDF

93. Alexandru, G., Graumann, J., Smith, G.T., Kolawa, N.J., Fang, R., and Deshaies, R.J. (2008). UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIFIa turnover. Cell 134, 804-816. Abstract PDF/Suppl

94. Saha, A. and Deshaies, R. (2008). Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. Mol. Cell 32, 21-31. Abstract PDF/Suppl

95. Rodriguez-Gonzalez, A., Cyrus, K., Salcius, M., Kim, K., Crews, C.M., Deshaies, R.J., Sakamoto, K.M. (2008). Targeting steroid hormone receptors for ubiquitination and degradation in breast and prostate cancer. Oncogene 27, 7201-7211. Abstract PDF

96. Mohl DA, Huddleston MJ, Collingwood TS, Annan RS, Deshaies RJ. (2009). Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. J. Cell Biol. 184, 527-539. Abstract PDF/Suppl

97. Collins, G.A., Lipford, J.R., Deshaies, R.J., Tansey, W.P. (2009). Gal4 turnover and transcription activation (Brief Communication Arising). Nature 461, (8 October) E7.

98. Machida, K., Tsukamoto, H., Mkrtchyan, H., Duan, L., Dynnyk, A., Liu, H.M., Asahina, K., Govindarajan, S., Ray, R., Ou, J.H., Seki, E., Deshaies, R., Miyake, K., Lai, M.M. (2009). Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog. Proc. Nat. Acad. Sci. USA 106, 1548-1553. Abstract PDF

99. Oh, Y.M., Kwon, Y.E., Kim, J.M., Bae, S.J., Lee, B.K., Yoo, S.J., Chung, C.H., Deshaies R.J., Seol, J.H. (2009) Chfr is linked to tumour metastasis through the downregulation of HDAC1. Nat. Cell Biol. 11, 295-302. Abstract PDF

100. Deshaies, R.J. (2009). Drug discovery: Fresh target for cancer therapy (News & Views). Nature 458, 709-710. Abstract PDF

101. Deshaies, R.J. and Joazeiro, C.A.P. (2009). RING domain E3 ubiquitin ligases. Annu. Rev. Biochem. 78, 399-434. Abstract PDF. Author's note: I am pleased to provide you complimentary one-time access to my Annual Reviews article as a PDF file for your own personal use. Any further/multiple distribution, publication, or commercial usage of this copyrighted material requires submission of a permission request addressed to the Annual Reviews Permissions Department at

102. Kleiger G, Hao B, Mohl DA, Deshaies RJ. (2009). The acidic tail of the CDC34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCDC4. J. Biol. Chem. 284, 36012-36023. Abstract PDF

103. Kleiger, G., Saha, A., Lewis, S., Kuhlman, B., and Deshaies, R.J. (2009). Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates. Cell 139, 957-968. Abstract Full Text Supp

104. Pierce, N., Kleiger, G., Shan, S., and Deshaies, R.J. (2009). Detection of sequential ubiquitination on a millisecond time-scale. Nature 462, 615-619. Abstract PDF

105. Radhakrishnan, S.K., Lee, C.S., Young, P., Beskow, A., Chan, J.Y., and Deshaies, R.J. (2010). Transcription factor Nrf1 mediates the proteasome recovery pathway after proteasome inhibition in mammalian cells. Mol. Cell 38,17-28. Abstract PDF

106. Aghajan, M., Jonai, N., Flick, K., Fu, F., Luo, M., Cai, X., Ouni, I., Pierce, N., Tang, X., Lomenick, B., Damoiseaux, R., Hao, R., Del Moral, P.M., Verma, R., Li, Y., Li, C., Houk, K.N., Jung, M.E., Zheng, N., Huang, L., Deshaies, R.J., Kaiser, P., and Huang, J. (2010). Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat. Biotechnol. 28, 738-742. Abstract PDF

107. Collins, G.A., Gomez, T.A., Deshaies, R.J., and Tansey, W.P. (2010). Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast 27, 965-974. Abstract PDF

108. Tyrell, A., Flick, K., Kleiger, G., Zhang, H., Deshaies, R.J., and Kaiser, P. (2010). Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins. Proc. Natl. Acad. Sci. USA 107, 19796-19801. Abstract PDF

109. Deshaies, R.J., Emberley, E.D., and Saha, A. (2010). Control of cullin-ring ubiquitin ligase activity by Nedd8. Subcell. Biochem., 54, 41-56. Abstract PDF

110. Verma, R., Oania, R., Fang, R., Smith, G.T., and Deshaies, R.J. (2011). Cdc48/p97 Mediates UV-Dependent Turnover of RNA Pol II. Mol Cell. 41, 82-92. Abstract PDF

111. Chou, T.F., Brown, S.J., Minond, D., Nordin, B.E., Li, K., Jones, A.C., Chase, P., Porubsky, P.R., Stoltz, B.M., Schoenen, F.J., Patricelli, M.P., Hodder, P., Rosen, H., and Deshaies, R.J. (2011) Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc. Natl. Acad. Sci. U. S. A. [Epublished ahead of print, March 7, 2011]. Abstract PDF

112. Saha, A., Lewis, S., Kleiger, G., Kuhlman, B., and Deshaies, R.J. (2011). Essential role for ubiquitin-ubiquitin-conjugating enzyme interaction in ubiquitin discharge from Cdc34 to substrate. Mol. Cell 42, 75-83. Abstract PDF

113. Lee, J.E., Sweredoski, M.J., Graham, R.L., Kolawa, N.J., Smith, G.T., Hess, S., and Deshaies, R.J. (2011). The steady-state repertoire of human SCF Ubiquitin ligase complexes does not require ongoing Nedd8 conjugation. Mol Cell Proteomics 10, M110.006460. Abstract PDF

114. Chou, T.F. and Deshaies, R.J. (2011). Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system. J. Biol. Chem. 286, 16546-16554. Abstract PDF

115. Gomez, T.A., Kolawa, N., Gee, M., Sweredoski, M.J., and Deshaies, R.J. (2011) Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1. BMC Biol. 9, 33. Abstract PDF

116. Rabut, G., Le Dez, G., Verma, R., Makhnevych, T., Knebel, A., Kurz, T., Boone, C., Deshaies, R.J. and Peter, M. (2011). The TFIIH Subunit Tfb3 Regulates Cullin Neddylation. Mol Cell. 43, 488-495. Abstract PDF

117. Sohn CH, Lee JE, Sweredoski MJ, Graham RL, Smith GT, Hess S, Czerwieniec G, Loo JA, Deshaies RJ, Beauchamp JL. (2012). Click chemistry facilitates formation of reporter ions and simplified synthesis of amine-reactive multiplexed isobaric tags for protein quantification. J Am Chem Soc. 134, 2672-80. Abstract PDF

118. Haines, D.S., Lee, J.E., Beauparlant, S.L., Kyle, D.B., den Besten, W., Sweredoski, M.J., Graham, R.L., Hess, S., and Deshaies, R.J. (2012). Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking. Mol. Cell. Proteomics 11, M111.016444. Abstract PDF

119. Sohn, C.H., Agnew, H.D., Lee, J.E., Sweredoski, M.J., Graham, R.L., Smith, G.T., Hess, S., Czerwieniec, G., Loo, J.A., Heath, J.R., Deshaies, R.J., and Beauchamp, J.L. (2012). Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions. Anal. Chem. 84, 2662-2669. Abstract PDF

120. Besten Wd, Verma R, Kleiger G, Oania RS, and Deshaies, RJ. (2012). NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat Struct Mol Biol. 19, 511-516. Abstract PDF

121. Emberley, E.D., Mosadeghi, R., and Deshaies, R.J. (2012). Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-Fbox and substrate, and CSN inhibits deneddylated SCF by a non-catalytic mechanism. J. Biol. Chem. E-published ahead of print, Jul 5. Abstract PDF

122. Oh, Y., Chang, K.J., Orlean, P., Wloka, C., Deshaies, R., and Bi, E. (2012). Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol. Biol. Cell. 23, 2445-2456. Abstract PDF

Ray Deshaies's Graduate School and Postdoctoral Publications

1. Fish, L.E., Deshaies, R., and Jagendorf, A.T. (1983). A Mg2+ requirement for rapid ATP-driven protein synthesis by intact pea chloroplasts. Plant Sci. Lett. 31, 139-146.

2. Deshaies, R.J., Fish, L.E., and Jagendorf, A.T. (1984). Permeability of chloroplast envelopes to Mg2+. Effects on protein synthesis. Plant Physiol. 74, 956-961.

3. Deshaies, R.J., and Schekman, R. (1987). A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J. Cell Biol. 105, 633-645.

4. Deshaies, R.J., Koch, B.D., Werner-Washburne, M., Craig, E.A., and Schekman, R. (1988). 70 kD stress protein homologues facilitate translocation of secretory and mitochondrial precursor polypeptides. Nature 332, 800-805.

5. Bohni, P.C., Deshaies, R.J., and Schekman, R. (1988). SEC11 is required for signal peptide processing and yeast cell growth. J. Cell Biol. 106, 1035-1042.

6. Deshaies, R.J., Koch, B.D., and Schekman, R. (1988). The role of stress proteins in membrane biogenesis. Trends Biochem. Sci. 13, 384-388.

7. Deshaies, R.J., Kepes, F., and Bohni, P.C. (1989). Genetic dissection of the early stages of protein secretion in yeast. Trends Genet. 5, 87-93.

8. Deshaies, R.J., and Schekman, R. (1989). SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum. J. Cell Biol. 109, 2653-2664.

9. Rothblatt, J.A., Deshaies, R.J., Sanders, S.L., Daum, G., and Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell Biol. 109, 2641-2652.

10. Deshaies, R.J. and Schekman, R. (1990). Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Mol. Cell. Biol. 10, 6024-6035.

11. Deshaies, R.J., Sanders, S., Feldheim, D., and Schekman, R. (1991). Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature 349, 806-808.

12. Stirling, C.J., Rothblatt, J., Hosobuchi, M., Deshaies, R., and Schekman, R. (1992). Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell 3, 129-142.

13. Esnault, Y., Blondel, M.-O., Deshaies, R.J., Schekman, R. and Kepes, F. (1993). The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum. EMBO J. 12, 4083-4093.

14. Booher, R.N., Deshaies, R.J. and Kirschner, M.W. (1993). Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J. 12, 3417-3426. Abstract

15. Li, J.J. and Deshaies, R. J. (1993). Exercising self-restraint: discouraging illicit acts of S and M in eukaryotes. Cell 74, 223-226.

16. Deshaies, R.J., Chau, V., and Kirschner, M.W. (1995). Ubiquitination of the G1 cyclin Cln2p by a Cdc34p-dependent pathway. EMBO J. 14, 303-312. Abstract

17. Deshaies, R.J. and Kirschner, M.W. (1995). G1 cyclin-dependent activation of p34CDC28 in vitro. Proc. Natl. Acad. Sci. USA 92, 1182-1186. Abstract

18. Gerber, M.R., Farrell, A., Deshaies, R.J., Herskowitz, I. and Morgan, D.O. (1995). CDC37 is required for association of the protein kinase CDC28 with G1 and mitotic cyclins. Proc. Natl. Acad. Sci. USA 92, 4651-4655. Abstract

19. Banerjee, A., Deshaies, R.J., and Chau, V. (1995). Characterization of a dominant negative mutant of the cell cycle ubiquitin-conjugating enzyme Cdc34. J. Biol. Chem. 270, 26209-26215. Abstract