Caltech Bi 211 Fall 1999

Revised 11/9/99
Course announcement
Tentative Schedule
Requirements
Session 7
Tuesday
11/9
7:00 PM
Transporters and Pumps
 Carrie Shilyanksy
Sachiko Murase

Readings in Hille

None.  Choose one or two of the reviews, below.

Additional Required Reading

Neurotransmitter Transporter
Lu, C. C. and D. W. Hilgemann 1999. GAT1 (GABA:Na+:Cl-) Cotransport Function. Steady state studies in giant xenopus oocyte membrane patches. J Gen Physiol. 114:429-444.
 Reprint   Local reprint
Lu, C. C. and D. W. Hilgemann 1999. GAT1 (GABA:Na+:Cl-) Cotransport Function. Kinetic studies in giant xenopus oocyte membrane patches. J Gen Physiol. 114:445-458.
Reprint Local reprint
Hilgemann, D. W. and C. C. Lu 1999. GAT1 (GABA:Na+:Cl-) Cotransport Function. Database reconstruction with an alternating access model. J Gen Physiol. 114:459-476.
ReprintLocal reprint

Fairman, W. A., Vandenberg, R. J., Arriza, J. L., Kavanaugh, M. P., and Amara, S. G. (1995). An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature 375, 599-603.
Transporters and channels are very similar indeed.

CFTR
Zeltwanger, S., F. Wang, G. T. Wang, K. D. Gillis and T. C. Hwang 1999. Gating of cystic fibrosis transmembrane conductance regulator chloride channels by adenosine triphosphate hydrolysis. Quantitative analysis of a cyclic gating scheme. J Gen Physiol. 113:541-54.
 ReprintLocal reprint
Weinreich, F., J. R. Riordan and G. Nagel 1999. Dual effects of ADP and adenylylimidodiphosphate on CFTR channel kinetics show binding to two different nucleotide binding sites. J Gen Physiol. 114:55-70.
 ReprintLocal reprint
Two papers that show how to reason from kinetics and single-channel measurments to gating mechanisms involving ATP hydrolysis.

Tabcharani, J. A., P. Linsdell and J. W. Hanrahan 1997. Halide permeation in wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels. J Gen Physiol. 110:341-54.
 Reprint
Linsdell, P., J. A. Tabcharani, J. M. Rommens, Y. X. Hou, X. B. Chang, L. C. Tsui, J. R. Riordan and J. W. Hanrahan 1997. Permeability of wild-type and mutant cystic fibrosis transmembrane conductance regulator chloride channels to polyatomic anions. J Gen Physiol. 110:355-64.
 Reprint
Linsdell, P., J. A. Tabcharani and J. W. Hanrahan 1997. Multi-Ion mechanism for ion permeation and block in the cystic fibrosis transmembrane conductance regulator chloride channel. J Gen Physiol. 110:365-77.
 Reprint
Three papers showing the state of the art at defining a channel lumen: inorganic ions; organic anion "sizing"; and anomalous mole fraction effects.

The Na-K-ATPase
Canfield, V. A. and R. Levenson 1998. Domain swapping between Na,K- and H,K-ATPase identifies regions that specify Na,K-ATPase activity. Biochemistry. 37:7509-16.
 Paper    Local source

Reviews

W. R. Harvey and C. L. Slayman (1994). Coupling as a way of life. J. Exp. Biol.196: 1-4.
Slayman (1994. Metamorphosis of research on ion-coupled metabolite transport. J. Exp. Biol 196: 139-144.
Maloney, P. C.1994 Bacterial and plant transporters. J Exp. Biol. 196: 439-442.
Nelson, N. 1994. Energizing porters by proton-motive force. J. Exp. Biol. 196: 7-13.

Lester, H. A., Y. Cao and S. Mager 1996. Listening to neurotransmitter transporters. Neuron. 17:807-10.
 Reprint

Rakowski, R. F., D. C. Gadsby and P. De Weer 1997. Voltage dependence of the Na/K pump. J Membr Biol. 155:105-12
 Reprint

Seal, R. P. and S. G. Amara 1999. Excitatory amino acid transporters: a family in flux. Annu Rev Pharmacol Toxicol. 39:431-56.
 Reprint

Gadsby, D. C. and A. C. Nairn 1999. Control of CFTR channel gating by phosphorylation and nucleotide hydrolysis. Physiol Rev. 79:S77-S107.
 Reprint

Blanco, G. and R. W. Mercer 1998. Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol. 275:F633-50.
 Reprint