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Setup
A setup for TIRF imaging and caged-calium flash experiments (see the figure above)
Protocols
Genotyping of complexins transgenic mice (DNA extraction, PCR & gel electrophoresis)
Mouse chromaffin cell culture (adult mice and new born mice)
Immunocytochemistry
Western Blot
Procedure of making Carbon Fiber Electrodes (CFE)
Procedure of caged-calcium experiments
Lab solutions for electrophysiology
Semliki Forest Virus (SFV) Expression System (provided by
Dr. Rod Bremner
, Toronto, Canada)
Igor Macros and Functions (Electrophysiology Data Analysis)
Igor function for membrane capacitance and calcium currents analysis (depolarization stimulus)
Igor macro for amperometry data analysis (with R Chow and K Gajeera)
Igor function for triple-components exponential fit (updated on 12/13/2006)
Igor function for RAMP data analysis
Patcher's Power Tools
(provided by Max Planck Institute for Biophysical Chemistry, Germany)
Online Analysis
Primer design
(provided by Whitehead Institute for Biomedical Research, MIT)
Manipulate and Display a DNA Sequence
(provided by Colorado State University)
Mann-Whitney U Test
(provided by
Dr. Leon Avery
, Dallas)
© 2008 Haijiang Cai
