- Protein Purification, ELISAs, Cell Culture, Confocal Microscopy, Image Analysis, SQL & HTML
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Bachelor of Arts in Biology University of California, Santa Cruz 1986
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TISSUE CULTURE & MOLECULAR BIOLOGY -
Primary CNS cells, CHO, PC12, HeLa, MDCK, etc. cell lines, in situ hybridization, ICC, radiation techniques, cloning cylinders, cryopreservation, Maxi-prep, Mini-prep, G418 selection, operation of ABI 310 DNA sequencer, PCR equipment, plasmid purification by density gradient ultracentrifugation w/ Ethidium Bromide
MICROSCOPY - Confocal microscopy, photomicroscopy, time-lapse videomicroscopy, ICC, in situ hybridization, histology, EM darkroom, TEM, SEM, biological imaging & photography, multiphoton microscopy
BIOCHEMISTRY & MICROBIOLOGY - Protein purification & assays, chromatography, All Standard microbiological techniques, Enzyme Linked Immunosorbent Assay (ELISA), Large scale: Cell Pharm, Fermacell, Sharples, French Press
NEUROPHYSIOLOGY & ANIMAL SURGERY - Microdissection of brain and ocular structures (rodent & teleost), stereotaxic brain surgery (rodent), Iontophoretic drug applications to neurite growth cones, necropsy, retina explant culture COMPUTER - MS-Office, Excel, Access, HTML, PC upgrades, UNIX, vi, SPSS-PC, NIH-Image
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1998-Present -- Research Technician, California Institute of Technology
1998-98 -- Instrument Technician, California Institute of Technology
1997-97 -- Research Technician, Web Page Designer, School of Medicine, USC
1995-97 -- Naturalist - Cabrillo Marine Aquarium, Los Angeles
1992-95 -- Research Technician III/supervisor - Univ. Southern California
1989-91 -- Staff Research Associate II - University of California, Irvine
1988-89 -- Teaching Assistant - Biology, University of Hawaii, Manoa
1986-87 -- Laboratory Assistant II - University of California, Irvine
1984-85 -- Student Computer Consultant - University of California, Santa Cruz
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E-mail: hank@caltech.edu
Web Site: http://flzhgn.home.mindspring.com/indexc.htm
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