Caltech Flow Cytometry Facility

020 Kerckhoff | 626.395.3998

Equipment

The Caltech Flow Cytometry Facility is equipped with two research grade flow cytometer/cell sorters capable of sorting 3000 - 10000 events/second based on multiparameter analysis of the cells characteristics (size, density, and fluorescence intensities of multiple colors). In addition, one non-sorting analyzer (FACSCalibur) and a work station are available to researchers for self-service analysis provided that they demonstrate competence to use the analyzer and have taken training provided by the facility.

A completed Biosafety Form must be submitted prior to operator-run appointments or self-service experiments.

BD Biosciences FACSAria Flow Cytometer Cell Sorter

• capable of 9 color analysis and sorting
• excitation wavelengths of 407 nm, 488 nm, 633 nm
• robotic arm for sorting into a various plates and tubes including 96-well PCR format
• color data output with color printer for multiple color gating of data
• PC computer platform
• off-line networking
• high speed sorting up to 10K events/second with excellent recovery
• 4-way sort collection

iCyt Mission Technology Reflection Cell Sorter

• resident in Baker Bioguard III biosafety cabinet under BSL2 conditions
• capable of 9 color analysis and sorting
• excitation wavelengths at 355 nm (UV), 407 nm, 488 nm, 561 nm, 633 nm
• sample deposition station for sorting into a variety of plates and tubes including 96 well format for plates and 4 way tube sorting
• PC platform with color data output with color printer for multiple color gating using WinList from Verity Software House
• high speed sorting > 10K events/second with excellent recovery
• remote viewing of sort windows

FACSCalibur four color analyzer

• equipped with both an air-cooled Argon laser and a red diode laser outputting at 635nm
• Macintosh G4 computer platform, with an off-line networked workstation