Bjorkman Lab - Protocols
All protocols viewable via pdf

* MAMMALIAN CELLS IN USE AT PEC
Michael's group meeting presentation

293 cell transfection

* BACTERIAL EXPRESSION
PI-PLC purification
Inclusion body preparation
Ben's E.coli expression and renaturing

* BACULOVIRUS
Baculovirus-transfection
Sf9 Spinner Media Recipe

* Biacore
Covalent oriented coupling
Biacore instructions

SPR protocols

* CALCULATING KDs
Equilibrium Dialysis
Scatchard Analysis
B27 binding assay

* CLONING
Pouring LB-Agar+Antibiotic Plates
Gibson Cloning
Site-Directed Mutagenesis
Restriction Cloning
Preparation of electocompetent E. coli
How to label DNA tubes
PJB's cloning hints
Cloning tips by Astrid
Lance's subcloning procedures
Site-directed mutagenesis protocol
bacterial recombination cloning protocol
competent cell prep (6/2012)
tranformation (6/2012)
Z-competent Cell Transformation
2-stage site-directed mutagenesis

* CRYSTALLISATION
Siliconized coverslips

* ELISA
ELISA
Anti FcRn ELISA
Polyreactivity assay
Dot Blot ELISA

* IMMUNOPRECIPITATION
Immunoprecipitations (FcRn)
Immunoprecipitations (FcRn) short
Immunoprecipitations (HFE)

* IMMUNOSTAINING/CELL SORTING
Immunostaining of cells
FcRn sorting

* SPINNING DISK
Laser calibration

* PROTEIN ELECTROPHORESIS
TCA precipitation protocol
Silver staining of SDS-PAGE
SDS-PAGE and Western blot
Western blot

* PROTEIN PURIFICATION
How to use the AKTA
1G3 Column-short
1G3 Column for FcRn mutants
Anti-FcRn mAb
Antibody by Protein G
HFE - short protocol
HFE - full protocol
TFR (his-tagged)
B27
FcRn
Antibodies
kd/cb2m
kd/mb2m
T cell receptor
UL18 from insect-cell media
Purification of His-tagged proteins
Purification of StrepII-tagged proteins

nickel vs. cobalt columns
Fca Purification Protocol
Concentrator instructions
Recharging Ni beads
Osmotic Shock and TRX-htt purification
Fab production and purification
PSMA purification
anti-FLAG purification

* TISSUE CULTURE
Tissue Culture Guidelines

Maintenance of HFE producing cells
Freezing adherent mammalian cells
Harvesting proteins Chinese Hamster Ovary cells
Splitting adherent mammalian cells

 

* TRANSFECTION
Maria's Maxiprep
Blaise’s Finishing Maxipreps without Finalizers
Lipofection Procedure
Ca-P Transfection MDCK
Stable transfection of CHO-cells
PCR genes from cells

*USDA - APHIS REGULATIONS
APHIS regulations letter
U.S. Customs Protein Import

*THERMOFLUOR ASSAY
Thermofluor Assay protocol

* MISC
Keeping a lab book
Pichia MeOH induction screening
BMMY, BMGY recipes
Metabolic labeling of cells
Acid elution tips
Christina's notes on FcRn constructs
ZipTip
Preparing figures for publication
Molecular movies
Polyclonal Antiserum Production

* WRITING A PAPER
Paper/proposal writing guidelines

*YUMMIES
Jenny's Beef Protocol
Beth's Banana Bread


bjorker home page: http://www.its.caltech.edu/~bjorker/