Bjorkman Lab - Monoclonal Antibodies

We have submitted most of our hybridomas to the American Type Culture Collection (ATCC). Below is a brief summary of each antibody and a link to the ATCC page that describes how to order each hybridoma and how to grow it.

If you need any other reagents from our lab, please send an e-mail to Pamela Bjorkman - (bjorkman[at]caltech.edu) with:

1) A specific description of what you want
2) A complete shipping address including your phone number
3) Your Federal Express number for shipping charges

Please note that we cannot give out cell lines or expression vectors that include the glutamine synthetase (GS) gene unless you have a license to use the GS expression system. (These include cell lines and vectors for expression of FcRn, HFE, and Fc fragments.) We obtained our license from Celltech Therapeutics, but Celltech has since sold the intellectual property associated with the GS expression system to Lonza Biologicals plc. Those interested in obtaining a license should contact either Karen Fallen, Commercial Licensing Manager, or Isabella Sharratt, GS Administrative Assistant, at GSLonza@lonza.com. Their address is 228 Bath Road, Slough, Berkshire SL1 4DX; telephone number is +44 1753 777000; fax +44 1753 777001.

Antibodies against cytomegalovirus class I MHC homologs (See Fahnestock, M.L., J.L. Johnson, R.M.R. Feldman, J.M. Neveu, W.S. Lane, and P.J. Bjorkman (1995) The MHC class I homolog encoded by human cytomegalovirus binds endogenous peptides. Immunity 3: 583-590 andChapman, T.L. and P.J. Bjorkman (1998) Characterization of a murine cytomegalovirus class I MHC homolog: Comparison to MHC molecules and to the human cytomegalovirus MHC homolog. J. Virol. 72: 460-466.

Hybridoma Name

Antigen recognized

Antigen
injected

ELISA

Western blot

IP & immunoaffinity chromatography

live cell staining

ATCC number

ATCC
Link

10C7

UL18

purified UL18/B2m heterodimers
+
+
+
+

CRL-2430

ATCC

15C6

m144

gel slice of m144
+
+
+
+

CRL-2431

ATTC

19G4

m144

purfied m144/human B2m heterodimers
+
-
-
?

CRL-2432

pending approval
.

Antibodies against FcRn (see Raghavan, M., L.N. Gastinel, M.Y. Chen, and P.J. Bjorkman (1994) Investigation of the interaction between the lass I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315.

Hybridoma Name

Antigen recognized

Antigen
injected

ELISA

Western blot

IP & immunoaffinity chromatography

live cell staining

ATCC number

ATCC
Link

1G3

FcRn

purfied rat. FcRn/B2m heterodimers
+
+
+
+

CRL-2434

ATCC

2G3

FcRn

purfied rat FcRn/B2m heterodimers
+
+
-
-

CRL-2435

ATCC

4C9

rat B2m

purfied rat FcRn/B2m heterodimers
+
+
+
(IP B2m alone and FcRn/B2m heterodimers)
+

CRL-2437

ATCC
.

Antibodies against HFE (see Lebrón, J.A.*, M.J. Bennett*, D.E. Vaughn, A.J. Chirino, P.M. Snow, G.A. Mintier, J.N. Feder and P.J. Bjorkman (1998) Crystal Structure of the Hemochromatosis Protein HFE and Characterization of its Interaction with Transferrin Receptor. Cell 93:111-123. (* these authors contributed equally to the work) andLebrón, J.A., H. Shen, P.J. Bjorkman and S. Ou (1999) Tolerization of adult mice to immunodominant protein before monoclonal antibody production. J. Immunol. Meth. 222/1-2: 59-63.

Hybridoma Name

Antigen recognized

Antigen
injected

ELISA

Western blot

IP & immunoaffinity chromatography

live cell staining

ATCC number

ATCC
Link

3A5

HFE

purfied human HFE/B2m heterodimers
+
+
(weak)
++
-

CRL-2440

ATCC

1C3

HFE

purfied human HFE/B2m heterodimers
+
-
+++
-

CRL-2441

ATCC

2A11

HFE

purfied human HFE/B2m heterodimers
+
-
+
-

CRL-2442

ATCC

2B7

HFE

purfied human HFE/B2m heterodimers
+
-
+
-

CRL-2443

ATCC

2A5

HFE

purfied human HFE/B2m heterodimers
+
+
(weak)
++
-

CRL-2444

ATCC

Antibodies against human Zn-alpha2-glycoprotein (ZAG) (see Sánchez, L.M., C. López-Otín and P.J. Bjorkman (1997) Biochemical characterization and crystallization of human Zn-alpha2-glycoprotein, a soluble class I MHC homolog. Proc. Natl. Acad. Sci. USA 94: 4626-4630.

The anti-ZAG mAbs are now available from Santa Cruz Biotechnology, Inc.:

Contact:
Santa Cruz Biotechnology, Inc.
2161 Delaware Avenue
Santa Cruz, California 95060 U.S.A.
Toll Free: 1-800-457-3801
Phone: 831-457-3800
Fax: 831-457-3801
scbt@scbt.com
http://www.scbt.com/

link to purchase: ZAG (1D4): sc-13585