LAB RESPONSIBILITIES
as of 10/09
Alex order LN2 tanks for TC cell dewar, -80°C chest freezer and crystallography, shaking incubators, bacterial incubators
Anthony SMART system, Biacore (backup), computers
Beth safety officer, replace radiation badges and rings, general TC maintenance (aliquot heat-inactivated FBS, dialyzed heat-inactivated FBS, goat serum, Penn/Strep, L-glutamine, MSX, TC hoods, TC incubators), LN2 cell dewars for tissue culture cell lines, TC cell line notebook/inventory, Bjorklab online inventories (cell lines, constructs, vectors, primers), Addgene, animal protocols, order lab supplies (TechMart)
Caleb LB/antibiotic plates, Water baths (cleaning/filling – 16°C, 37°C, 42°C, 65°C), organize lab primer and vector stocks
Fan organize/clean cold room crystal area in Jensen lab cold room (Broad, 335), PCR machines, pH meter, acid/base dilutions, pH standards, gel filtration standards
Galina spinning disk microscope (in Beckman Institute)
Josh Biacore, radiation safety officer, organize lab primer and vector stocks
Lynn order lab supplies (HHMI), track orders, check-in and date received packages, purchase ethanol, stock shelves with general lab supplies (tubes, plates, filters, syringes, needles, pipets, pipet tips, office supplies, etc.), autoclave and put away clean glassware, autoclave tips, tubes and bottles, service contacts/renewals, recycling, liaison with sales representatives
Maria autoclave bacterial waste
Mark High pressure freezer, AFS (freeze substitution machine), microtome, tissue culture microscopes, computers
Marta Pamela's assistant, group meeting assignments, Bjorklab web design/update, Bjorklab mailing list, Kronos time keeper, lab roster, annual report coordinator, printer/copier service, PTA/funding coordinator, email reminders (work updates, group meeting food, birthday celebrations, coordinates meetings with Pamela)
Matt Weekly manual backup of data from AKTAs, Biacore, DLS and XL-1
Paola pipetmen and pipet aids, order lab supplies (TechMart), 70% ethanol (general lab), TAE, destain, 1X and 10X Laemmli buffers (both with and without SDS), Nanodrop and Cary spectrophotometers, ELISA plate readers, LN2 cell dewars for tissue culture cell lines (backup), organize lab primer and vector stocks
Pavle Mosquito robot, Fluidigm system
Pri clean and organize weighing area, organization/ordering of plasmid miniprep, PCR cleanup and gel purification kits, general TC maintenance (aliquot heat-inactivated FBS, dialyzed heat-inactivated FBS, goat serum, Penn/Strep, L-glutamine, MSX, TC hoods, TC incubators), order lab supplies (TechMart), Electroporator
Rachel Milli-Q water system (maintenance and filling carboys), organization/ordering of plasmid maxiprep kits, organization/ordering of restriction enzymes, mutagenesis kits, electro-competent and chemically-competent cells, order lab supplies (TechMart), Amicon concentrator, 4°C AKTA, centrifuges (backup)
Ron room temperature AKTA, Millipore concentrator, lab cameras, organize/clean room temperature crystallization area, stock general crystallization supplies, crystallography microscope, LN2 dewar for storing crystals
Yongning centrifuges