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Hours
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Mon
3:00-4:00 pm (Church 68)
Fri
2:00-4:00 pm (Church 60)
Lab:
Church 68
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Student
group list
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Preliminary
list available here
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Syllabus
(updated 10/02)
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week
1
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10/02:
Administrative session
Training
sessions to be scheduled with Ned
Download
LSM310 manual
Download
LSM410 manual
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week
2
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10/05:
Assignement #1
2D
CLSM imaging of fixed samples
Download
assigment #1
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10/09:
Q&A session + Introduction to lasers (John Choi)
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week
3
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10/12:
Assignement #2
3D
and multi-channel CLSM imaging of fixed samples
Download
assigment #2
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10/16:
Student presentations (Assignement #1)
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week
4
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10/19:
Assignement #3
Live
imaging I: fly embryos
Download
assigment #3
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10/23:
Student presentations (Assignement #2)
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week
5
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10/26:
Assignement #4
Live
imaging II: zebrafish embryos
Download
assigment #4
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10/30:
Student presentations (Assignement #3)
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week
6
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11/02:
Assignement #5
Image
analysis
Download
assigment #5
Download
Sample Images for assigment #5
Final
Project proposal due!
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11/06:
no class (Biology division retreat)
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week
7
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11/09:
Feedback on final project proposal
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11/13:
Student presentations (Assignement #4)
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week
8
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11/16:
Final project Q&A
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11/20:
Student presentations (Assignement #5)
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week
9
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11/23:
Final project Q&A
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11/27:
no class (Thanksgiving)
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week
10
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11/30:
Final project Q&A
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12/04:
Student presentations (Final project)
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Grading
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50%
Weekly lab assignments
50%
Final project
see
the grading policy here
see
the Final Project
rules
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Office
Hours
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By
appointment:
Willy
Supatto, supatto AT caltech.edu, x2863
Edward M. Perkins, ned AT caltech.edu
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Obtaining
keys to Church 68
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Contact:
Mary Marsh, 110 Kerckhoff, marshm AT caltech.edu, x6444
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Microscope
Calendar Access
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Students
need to access the calendar system for microscope and Imaris station
reservations:
- go to: http://calendar.bic.caltech.edu/.
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click on "New users please Register here".
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fill in required information.
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use "Caltech Labs" as affiliation
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Request access to 4 Calendars: (1) Bi227 CLASS 310, (2) Bi227 CLASS
410 (3) Bi227 CLASS 410 NLO, and (4) Bi 227 IMARIS.
This
will send an email to request official access to the calendars.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without reserving
it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete your reservation
on calendar.
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Try to avoid imaging late at night, since no one will be around to
help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris
(Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy
from the Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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