Bi 177: Principles of Modern Microscopy California Institute of Technology - Fall 2010 Prof. Scott E Fraser TAs: Danielle Bower, dvbrown AT caltech.edu, Jessica Ricci, jricci AT caltech.edu
Hours Mon, Wed, Fri 1:00-2:15 pm Broad 100 Lecture Hall
Lecture schedule (MAY BE UPDATED!) Oct 06 Lecture 01: Basic opticsSyllabus
Homework 1, Due 10/8/10 (beginning of class)
Oct 08 Lecture 02: Design and layout of real microscopes
Homework 2, Due 10/11/10
Oct 11 Lecture 03: Wide Field Fluorescence Microscopy
Oct 13 Lecture 04: Lasers (physics, types, functions)
Oct 15 Lecture 05: Widefield vs laser scanning microscopy: adding the 3rd dimension
Homework 3, Due 10/20/10
Oct 18 Lecture 06: Practical considerations in a confocal microscope
** By 1pm email TAs the paper you will review for project 1
Oct 20 Lecture 07: Techniques for enhancing intrinsic contrast
** TAs will approve your paper selection or request a new one
Oct 22 Lecture 08: Resolution, contrast, and detection Nov 01 Lecture 09: Multiple labeling and multi-spectral imaging
** Draft of project 1 paper due at the beginning of class! Exchange with peers to review.
Nov 03 Lecture 10: FRET
**Comments on your peer's paper due at beginning of class!
Nov 05 Lecture 11: Quantitative Fluorescence Microscopy Nov 08 Lecture 12: Fast fluorescence and lightsheet microscopy
**Final revised project 1 paper due at beginning of class!
Nov 10 Lecture 13: Electron microscopy and tomography (Grant Jensen)
**By 1pm email us the paper you will review for project 2.
Nov 15 Eric Betzig's seminar on super-resolution microscopy Nov 17 Lecture 14: MRI and MR microscopy (Russ Jacobs) Nov 19 Lecture 15: Emerging imaging techniques I (NSOM, SIM, AFM, OPT, OCT)
** Draft of project 2 paper due at the beginning of class. Exchange for peer review.
Nov 22 Lecture 16: Emerging imaging techniques II (STED, 4pi, PALM/STORM) Nov 24 **Complete peer review and exchange comments.
Dec 03 **Final, revised project 2 due by 1pm!
Please turn in to Danielle (Brown OR Bower)'s mailbox in the BI lobby:
1) your first draft of paper #2,
2) the peer comments you received, and
3) your final draft,
all paper-clipped together.
Coursework Reading 6 Simple problem sets Paper reading project (2 per term) No midterm or final exam
Grading Grades based on the Homework and the Projects. Homework, Project 1, Project 2 are given equal weight. Late homework assignments are not accepted. Score can be dropped with medical or Dean's excuse.
Auditors and non-traditional students Auditors are welcome to take the Course, but are required to do the homework problem sets and reading.
Links Bi177 & Bi227 website Microscope Primer Microscopy from the Very Beginning (Zeiss) Olympus Microscopy Primer