Bi 177: Principles of Modern Microscopy California Institute of Technology - Fall 2008 John Choi and Willy Supatto TA: Judith Su
Hours Mon and Wed 1:00-2:30 pm Broad 100 Lecture Hall
Lecture schedule (updated 10/12) Sept 29 Lecture 01: Basic optics (JC) >download homework (due Oct 01) Oct 01 Lecture 02: Optics of real microscopes (JC) >download homework (due Oct 13) note: Imaris software presentation on Oct 03 (2-3pm in Broad 100) Oct 06 Science Writing Workshop with Steven Youra (Project 1) Oct 08 Science Writing Workshop with TA (Project 1) Oct 13 Lecture 03: Widefield light microscopy (JC) >download homework (due Oct 22) Oct 15 Lecture 04: Contrast techniques (JC) Oct 20 Lecture 05: Fluorescent microscopy (JC) Oct 22 Lecture 06: Electron Microscopy and Tomography (Grant Jensen) >download homework (due Oct 31) Oct 27 Lecture 07: Confocal microscopy I (JC) Oct 29 Lecture 08: Confocal microscopy II (JC) >download the reading Nov 03 Lecture 09: Multiphoton microscopy I (2PEF) (WS) Nov 05 Lecture 10: Multiphoton microscopy II (SHG, THG, CARS) (WS) Nov 10 Science Writing Workshop with TA (Project 2) Nov 12 Lecture 11: MRI and MR microscopy (Russ Jacobs) Nov 17 Lecture 12: Motion and fast imaging (WS) Nov 19 Lecture 13: Labeling and multi-spectral imaging (WS) Nov 24 Lecture 14: Quantitative fluorescence microscopy (FLIM, FRET, FRAP, FCS) (WS) Nov 26 Lecture 15: Digital imaging I: image formation, visualization and processing (WS) Dec 01 Lecture 16: Digital imaging II: 3D-reconstruction, image restoration and analysis (WS) Dec 03 Lecture 17: Fluorescence nanoscopy (NSOM, TIRF, 4Pi, STED, nonlinear SIM, STORM-PALM-FPALM) (WS)
Reading Projects Read and study a published paper in a mainstream journal. Write a short report concerning this paper. The report should be ~5 pages long. Get pre-approval of TA on paper/topic. >download the Reading Project Grading Criteria
Assignment Schedule (updated 10/12) Reading Project 1: Oct 06 Look for journal article (due Oct 13) Oct 13 Write draft (due Oct 20*) Oct 20 Do peer-review (due Oct 27*) >download the Peer-Review Comment Sheet Oct 27 Write final version of paper (due Oct 31) Reading Project 2: Nov 10 Look for journal article (due Nov 17) Nov 17 Write draft (due Nov 24*) Nov 24 Do peer-review (due Dec 01*) Dec 01 Write final version of paper (due Dec 05**) *due at beginning of class ** in TA mailbox
Course Work Reading Simple problem sets Paper reading project (2 per term) No mid-term of final exam
Grading Grades based on the Homework and the Projects. Homework, Project 1, Project 2 are given equal weight. Late homework assignements are not accepted. Score can be dropped with medical or Dean's excuse.
Auditors and non-traditional students Auditors are welcome to take the Course, but are required to do the homework problem sets and reading.
Links Bi177 & Bi227 website Microscope Primer Microscopy from the Very Beginning (Zeiss) Olympus Microscopy Primer