Cell Type Specific Log10 QPCR Expression Values

Synonym	Cell Type	QPCR Expression	Normalized To	Data From
E2A	CD4	-0.92	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	CD4	-1.27	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	CD4_24-	-1.24	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	CD4_69-	-1.43	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	CD8	-1.24	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	CD8	-1.33	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	CD8_24-	-1.36	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	CD8_69-	-1.48	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN1	-0.96	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN1	-0.38	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN1	-0.66	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN1	-1.09	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN2	-0.81	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN2	-0.48	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN2	-0.7	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN2	-0.7	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN2	-0.42	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN2a	-0.6	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN2a	-0.52	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN3a	-0.81	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN3a	-0.66	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN3a	-0.43	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3a	-0.6	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3a	-0.46	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3a	-0.22	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN3b	-0.77	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN3b	-0.46	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN3b	-0.62	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3b	-0.72	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3b	-0.68	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DN3b	-0.46	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DN4	-1.08	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
E2A	DN4	-0.34	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
E2A	DP	-0.7	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DP	-0.8	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	DP69-	-1.34	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	DP69-	-0.85	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
E2A	GDT	-1.08	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
E2A	GDT	-1.05	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)

Effect of Key Gene Perturbations

Synonym	Perturbation	QPCR Expression	Relative To	Data From
E2A	BM	-0.48	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
E2A	Fliver	0.17	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
E2A	Mast	-0.49	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
.
E2A	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
E2A	LZ_noDL	-0.28	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
.
E2A	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
E2A	LZ_noDL	-0.24	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
.
E2A	LZ_DL	0	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
E2A	G3_DL	-0.1	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
E2A	KRRm_DL	-0.02	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
E2A	LZ_noDL	-0.24	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
E2A	G3_noDL	-0.29	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
E2A	KRRm_noDL	-0.23	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
.
E2A	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	DL_PU1	-0.36	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	DL_PU1	-0.29	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	DL_PU1	-0.22	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_LZ	-0.14	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_LZ	-0.43	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_LZ	-0.26	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_PU1	-0.8	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_PU1	-0.6	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
E2A	noDL_PU1	-0.33	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
.
E2A	Prmtv_LZ	0.21	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	Prmtv_LZ	-0.28	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	Prmtv_PU1	-0.16	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	Prmtv_PU1	-0.69	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_PU1	0.01	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_PU1	-0.41	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	ProT_PU1	-0.22	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_LZ	0.04	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_LZ	-0.27	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_LZ	-0.09	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_PU1	-0.29	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_PU1	-0.2	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
E2A	PreT_PU1	-0.23	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)

Evidence for Interactions

Upstream Node	Interaction Type	Target Node	Literature Evidence
PU1 activity	REPRESSES	E2A	Franco et al 2006 PNAS
Notch.CSL	PROMOTES	E2A	Franco et al PNAS 2006; Taghon et al 2007 Nat Immunol; both quite weak
E2A	PROMOTES	E2A antagonized by SCL.Tal1 (net E2A)	This bubble is net E2A escaping SCL/Tal1 heterodimer formation
E2A	REGULATES	E2A.E2A	by definition
E2A	PROMOTES	E2A.SCL	by definition