Cell Type Specific Log10 QPCR Expression Values

Synonym	Cell Type	QPCR Expression	Normalized To	Data From
SpiB	CD4	-4.08	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	CD4	-1.7	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	CD4_24-	-2.21	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	CD4_69-	-2.8	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	CD8	-3.92	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	CD8	-3.42	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	CD8_24-	-4.27	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	CD8_69-	-2.21	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Spib	DN1	-1.96	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Spib	DN1	-2.28	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN1	-1.25	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN1	-2.55	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB-1	DN1	-3.14	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-1	DN1	-3.51	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN1	-4.07	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN1	-4.68	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
SpiB	DN2	-1.05	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN2	-1.4	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN2	-1.92	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB-1	DN2	-3.56	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-1	DN2	-3.3	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN2	-2.9	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN2	-2.84	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
Spib	DN2a	-2.27	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Spib	DN2a	-2.03	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN2a	-2.51	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN2a	-3.8	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Spib	DN2b	-1.6	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Spib	DN2b	-1.57	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Spib	DN3a	-0.64	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Spib	DN3a	-0.7	bActin	Intrathymic T Cell Precursors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3a	-0.26	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3a	-0.33	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3a	-0.51	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3a	-0.29	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB-1	DN3a	-1.89	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-1	DN3a	-1.98	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN3a	-1.21	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN3a	-1.29	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
SpiB	DN3b	-1.19	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3b	-1.2	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3b	-1.64	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DN3b	-1.04	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB-1	DN3b	-2.68	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-1	DN3b	-3.95	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN3b	-2.33	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN3b	-2.95	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
SpiB-1	DN4	-4.4	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-1	DN4	-3.35	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN4	-3.08	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
SpiB-2	DN4	-2.6	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
SpiB	DP	-2.36	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DP	-2.72	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	DP69-	-2.92	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	DP69-	-1.96	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
SpiB	GDT	-2.62	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
SpiB	GDT	-2.09	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)

Effect of Key Gene Perturbations

Synonym	Perturbation	QPCR Expression	Relative To	Data From
SpiB	Prmtv_LZ	-0.22	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	Prmtv_LZ	-0.25	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	Prmtv_PU1	-1.05	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	Prmtv_PU1	-1.22	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_PU1	-0.52	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_PU1	-0.65	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	ProT_PU1	-0.43	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_LZ	-0.5	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_LZ	-0.43	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_LZ	-0.3	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_PU1	-1.13	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_PU1	-1.01	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
SpiB	PreT_PU1	-0.67	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)

Evidence for Interactions

Upstream Node	Interaction Type	Target Node	Literature Evidence
PU1 activity	REPRESSES	SpiB	Franco et al 2006 PNAS
DN3 Specific Regulator	PROMOTES	SpiB	David-Fung et al 2006 Immunol. Rev.; Taghon et al. 2006 Immunity; Lefebvre, J.M.et al.: Wiest, J Immunol. 2005 May 15;174(10):6184-94; Yui & Rothenberg 2004 J. Immunol.
pre-TCR signal	REPRESSES	SpiB	Taghon et al 2006 Immunity; Yui & Rothenberg 2004 J. Immunol.
Net E protein activity	PROMOTES	SpiB	Xu, W. and Kee, B. L. Blood. 2007 May 15;109(10):4406-14