Cell Type Specific Log10 QPCR Expression Values

Synonym	Cell Type	QPCR Expression	Normalized To	Data From
Lck	CD4	-0.64	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	CD4	-0.8	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	CD4_24-	-0.89	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	CD4_69-	-0.58	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	CD8	-0.58	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	CD8	-1.08	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	CD8_24-	-1.19	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	CD8_69-	-0.62	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DN1	-0.89	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN1	-1.12	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DN2	-0.04	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN2	-0.25	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN2	-0.27	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DN2a	-0.41	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN2a	-0.47	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DN3a	0.54	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3a	-0.01	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3a	0.08	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3a	0.26	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DN3b	0.04	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3b	-0.14	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3b	-0.24	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DN3b	-0.08	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DP	0	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DP	-0.04	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	DP69-	-0.4	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	DP69-	0	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
.
Lck	GDT	-0.62	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)
Lck	GDT	-0.66	bActin	Intrathymic T Cell Precursors vs. Myeloid Progenitors (Rothenberg, Moore, and Yui 2008, Nat. Rev. Immunology)

Effect of Key Gene Perturbations

Synonym	Perturbation	QPCR Expression	Relative To	Data From
Lck	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
Lck	LZ_noDL	-0.23	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
.
Lck	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
Lck	LZ_noDL	-0.29	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
.
Lck	LZ_DL	0	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Lck	G3_DL	-0.21	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Lck	KRRm_DL	-0.06	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Lck	LZ_noDL	-0.29	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Lck	G3_noDL	-0.59	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Lck	KRRm_noDL	-0.3	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
.
Lck	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	DL_PU1	-0.28	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	DL_PU1	-0.36	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	DL_PU1	-0.42	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_LZ	-0.26	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_LZ	-0.32	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_LZ	-0.11	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_PU1	-0.84	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_PU1	-0.76	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Lck	noDL_PU1	-0.66	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)

Evidence for Interactions

Upstream Node	Interaction Type	Target Node	Literature Evidence
PU.1 modulated by Notch.DL	REPRESSES	Lck	Franco et al PNAS 2006
Notch.CSL	PROMOTES	Lck	Taghon, Yui, Rothenberg 2007 Nat. Immunol.
Lck	PROMOTES	bubble	Bubble= intermediate TCR signaling complex