Cell Type Specific Log10 QPCR Expression Values

Synonym	Cell Type	QPCR Expression	Normalized To	Data From
Ikaros	DN1	-0.61	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
Ikaros	DN1	-0.36	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
Ikaros	DN2	-0.85	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
Ikaros	DN2	-0.56	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
Ikaros	DN3a	-0.93	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
Ikaros	DN3a	-0.53	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
Ikaros	DN3b	-1.12	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
Ikaros	DN3b	-0.22	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
.
Ikaros	DN4	-0.72	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)
Ikaros	DN4	-0.68	bActin	Immature Thymocyte Gene Expression Data (David-Fung et al. 2006 Immunological Reviews)

Effect of Key Gene Perturbations

Synonym	Perturbation	QPCR Expression	Relative To	Data From
Ikaros	BM	-1.04	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
Ikaros	Mast	-0.34	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
Ikaros	Fthymus	0.19	Thymus	GATA-3 Expression, Other Tissues (Taghon et al. 2007 Nat. Immunol)
.
Ikaros	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
Ikaros	LZ_noDL	0.09	LZ,DL	Notch-Delta interaction data (Franco et al. 2006 PNAS)
.
Ikaros	LZ_DL	0	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
Ikaros	LZ_noDL	-0.09	LZ,DL	Notch-Delta interaction data (Taghon et al. 2007 Nat. Immunol)
.
Ikaros	LZ_DL	0	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Ikaros	G3_DL	0.19	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Ikaros	KRRm_DL	0.13	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Ikaros	LZ_noDL	-0.09	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Ikaros	G3_noDL	0.1	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
Ikaros	KRRm_noDL	0.08	LZ,DL	Over Expressed GATA-3 (Taghon et al. 2007 Nat. Immunol)
.
Ikaros	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	DL_LZ	0	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	DL_PU1	-0.3	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	DL_PU1	-0.21	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	DL_PU1	-0.02	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_LZ	0.04	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_LZ	-0.13	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_LZ	0.36	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_PU1	-0.52	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_PU1	-0.41	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
Ikaros	noDL_PU1	-0.33	DL,LZ	PU.1-Notch signaling interaction data (Franco et al. 2006 PNAS)
.
Ikaros	Prmtv_LZ	-1.14	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	Prmtv_LZ	-0.28	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	Prmtv_PU1	-0.36	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	Prmtv_PU1	-0.99	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_LZ	0	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_PU1	-0.6	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_PU1	-0.7	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	ProT_PU1	-0.67	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_LZ	-0.02	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_LZ	-0.1	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_LZ	-0.13	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_PU1	-0.25	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_PU1	-0.72	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)
Ikaros	PreT_PU1	-0.57	Pro-T,LZ	PU.1 Effects per stage data (Franco et al. 2006 PNAS)

Evidence for Interactions

Upstream Node	Interaction Type	Target Node	Literature Evidence
PU.1 modulated by Notch.DL	REPRESSES	Ikaros	Franco et al PNAS 2006
High-level GATA-3	PROMOTES	Ikaros	Taghon, Yui, Rothenberg 2007 Nat. Immunol.
Ikaros	PROMOTES	Other B-cell Different'n Genes	based on its requirement in B development; Thompson, E.C.et al.: Merkenschlager, Immunity. 2007 Mar;26(3):335-44; Reynaud D.et al.: Singh, Nat Immunol. 2008 epub Jun 22.PMID: 18568028
Ikaros	PROMOTES	Negative inputs	M. Zarnegar unpublished