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Hours
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Mon 3:00-4:00
pm (Church 68)
Fri
2:00-4:00 pm (Church 60)
Lab:
Church 68
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Student
group list
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Preliminary
list available here
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Syllabus
(updated 10/02)
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week
1
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10/02:
Administrative session
Training
sessions to be scheduled with Ned
Download
LSM310 manual
Download
LSM410 manual
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week
2
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10/05:
Assignement #1
2D
CLSM imaging of fixed samples
Download
assigment #1
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10/09: Q&A
session + Introduction to lasers (John Choi)
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week
3
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10/12:
Assignement #2
3D
and multi-channel CLSM imaging of fixed samples
Download
assigment #2
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10/16: Student
presentations (Assignement #1)
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week
4
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10/19:
Assignement #3
Live
imaging I: fly embryos
Download
assigment #3
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10/23: Student
presentations (Assignement #2)
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week
5
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10/26:
Assignement #4
Live
imaging II: zebrafish embryos
Download
assigment #4
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10/30: Student
presentations (Assignement #3)
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week
6
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11/02:
Assignement #5
Image
analysis
Download
assigment #5
Download
Sample Images for assigment #5
Final
Project proposal due!
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11/06: no class
(Biology division retreat)
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week
7
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11/09: Feedback
on final project proposal
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11/13: Student
presentations (Assignement #4)
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week
8
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11/16: Final
project Q&A
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11/20: Student
presentations (Assignement #5)
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week
9
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11/23: Final
project Q&A
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11/27: no class
(Thanksgiving)
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week
10
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11/30: Final
project Q&A
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12/04: Student
presentations (Final project)
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Grading
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50% Weekly lab
assignments
50%
Final project
see
the grading policy here
see
the Final
Project rules
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Office
Hours
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By appointment:
Willy Supatto,
supatto AT caltech.edu, x2863
Edward M. Perkins, ned
AT caltech.edu
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Obtaining
keys to Church 68
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Contact: Mary
Marsh, 110 Kerckhoff, marshm AT caltech.edu, x6444
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Microscope
Calendar Access
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Students need
to access the calendar system for microscope and Imaris
station reservations:
- go to: http://calendar.bic.caltech.edu/.
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click on "New users please Register here".
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fill in required information.
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use "Caltech Labs" as affiliation
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Request access to 4 Calendars: (1) Bi227 CLASS 310, (2)
Bi227 CLASS 410 (3) Bi227 CLASS 410 NLO, and (4) Bi 227
IMARIS.
This
will send an email to request official access to the
calendars.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without
reserving it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete
your reservation on calendar.
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Try to avoid imaging late at night, since no one will be
around to help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris (Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy from the
Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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