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Hours
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Mon
3:00-4:00 pm (Chrurch 68)
Fri
2:00-4:00 pm (Chrurch 60)
Lab:
Church 68
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Student
group list
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available
here
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Syllabus
(updated 10/23)
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week
1
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09/29:
Administrative session
Training
sessions to be scheduled with Chris Waters
Download
LSM310 manual
Download
LSM410 manual
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10/03:
Imaris Seminar/training
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week
2
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10/06:
Assignement #1
2D
Imaging of fixed samples: widefield and CLSM microscopy
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10/10:
Q&A session
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week
3
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10/13:
Assignement #2
3D
and multi-channel CLSM imaging of fixed samples
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10/17:
Student presentations (Assignement #1)
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week
4
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10/20:
Assignement #3
Live
imaging I: fly embryos
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10/24:
Student presentations (Assignement #2)
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week
5
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10/27:
Assignement #4
Live
imaging II: zebrafish embryos
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10/31:
Student presentations (Assignement #3)
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week
6
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11/03:
Assignement #5
Image
analysis
>
Download
the Sample Images (zip file)
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11/07:
Student presentations (Assignement #4)
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week
7
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11/10:
Assignement #6
FRAP
experiment
Final
Project proposal due!
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11/14:
Student presentations (Assignement #5)
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week
8
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11/17:
Final project Q&A
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11/21:
Student presentations (Assignement #6)
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week
9
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11/24:
Final project Q&A
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11/28:
no class (Thanksgiving)
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week
10
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12/01:
Final project Q&A
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12/05:
Student presentations (Final project)
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Grading
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60%
Weekly lab assignments
40%
Final project
see
the grading policy here
see
the Final Project rules here
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Office
Hours
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By
appointment:
John
Choi, johnchoi AT caltech.edu, x4499
Willy
Supatto, supatto AT caltech.edu, x2863
Louisa
Liberman, louisa AT caltech.edu, x5952
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Obtaining
keys to Church 68
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Contact:
Mary Marsh, 110 Kerckhoff, marshm AT caltech.edu, x6444
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Microscope
Calendar Access
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Students
need to access the calendar system for microscope and Imaris station
reservations:
- go to: http://calendar.bic.caltech.edu/.
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click on "New users please Register here".
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fill in required information.
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use "Caltech Labs" as affiliation
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Request access to 3 Calendars: (1) Bi227 310 (2) Bi227 410 and (3)
Bi 227 IMARIS.
This
will send an email to request official access to the calendars.
Notes
about Scope Calendar reservations and usage:
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Never use the microscopes or the Imaris station without reserving
it first
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Be considerate of others
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reserve reasonable amounts of time
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If you cannot make it to your reserved time please delete your reservation
on calendar.
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Try to avoid imaging late at night, since no one will be around to
help if you have a problem.
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Links
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Bi177
& Bi227 website
Imaris
(Bitplane AG)
Bitplane
Training Center
Microscope
Primer
Microscopy
from the Very Beginning (Zeiss)
Olympus
Microscopy Primer
ImageJ
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