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Hours |
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Tuesday and Thursday 10:30 am - 12:00 pm Broad 200 Seminar Room |
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Lecture schedule (MAY BE UPDATED!)
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1/06 |
1) |
Introduction and course overview (Lecture
1) |
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1/08 |
2) |
Basic (Geometrical) Optics (Lecture
2) (Homework
1) (Reading
1) |
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1/13 |
3) |
Design and Layout of Real Microscopes (Lecture
3) (Homework
2) |
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1/15 |
4) |
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1/20 |
5) |
Illumination and Detectors for imaging (Lecture
5) (Laser
Handout) |
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1/22 |
6) |
Wide Field Fluorescent Microscopy & Detectors
Part 2 (Lecture
6) |
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1/27 |
7) |
Wide Field vs. Laser Scanning Microscopy:
Adding the 3rd Dimension (Lecture
7) (Homework
3) |
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1/29 |
8) |
Compromises between Resolution, Contrast and
Detection (Lecture
8) |
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2/03 |
9) |
Nomarski
Review and other contrast techniques (Lecture
9) |
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2/05 |
10) |
Scattering and imaging: Clearing, Adaptive optics
(Lecture
10) (Homework
4; due 2/17) (Adaptive
optics review) |
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2/10 |
11) |
Optogenetics
and Imaging Application (Ravi Nath,
Guest
Lecture) |
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(Project 1 due) |
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2/12 |
12) |
Quantitating Fluorescence, Multispectral Imaging,
FRET (Lecture
12) |
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2/17 |
13) |
Emerging Imaging Techniques (AFM, NSOM) (Lecture
13) (Homework
5; due 2/24) (The
good, the bad and the ugly) |
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2/19 |
14) |
Fluorescence fluctuation spectroscopy, FRAP and
molecular imaging (Lecture
14) (Gratton
FCS review) |
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2/24 |
15) |
Light sheet microscopy and fast imaging (Antti
Lignell, Guest
Lecture) |
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2/26 |
16) |
Emerging Imaging Techniques (TIRFM, STED,
PALM/STORM) (Lecture
16) (Homework6;
due 3/05) |
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3/03 |
17) |
Digital Images: formation, visualization,
processing, and analysis (Vikas
Trivedi, Guest
Lecture) |
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3/05 |
18) |
Emerging Imaging Techniques (SLM, holographic
imaging and more fast imaging) (Lecture
18) |
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3/10 |
19) |
Electron microscopy: Introduction, correlation
with confocals (Paul Webster, Guest
Lecture) (Reading) |
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Reading Projects |
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Read and study a published paper in a mainstream journal. Write a short report concerning this paper. The report should be ~5 pages long. Get pre-approval of TA on paper/topic.
2nd Project will be oral presentations presented
to the class on March 12th. |
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Coursework |
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Reading 6 Simple problem sets Paper reading project (2 per term) No midterm or final exam |
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Grading |
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Grades based on the Homework and the Projects. Homework, Project 1, Project 2 are given equal
weight. Late homework assignments are not accepted. Score can be dropped with medical or Dean's
excuse. |
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Auditors and
non-traditional students |
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Auditors are welcome to take the Course, and, if
they wish, they can do the homework problem sets and readings. |
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Links |
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Zeiss
Microscopy from the Very Beginning |
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