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Hours
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Mon, Wed, Fri 1:00-2:15 pm
Broad
100 Lecture Hall
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Lecture
schedule (MAY BE UPDATED!)
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Oct
06
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Lecture
01: Basic optics
Homework 1,
Due 10/8/10 (beginning of class)
Syllabus
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Oct
08
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Lecture
02: Design and layout of real microscopes
Homework 2,
Due 10/11/10
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Oct
11
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Lecture
03: Wide Field Fluorescence Microscopy
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Oct
13
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Lecture 04: Lasers (physics, types, functions)
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Oct
15
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Lecture 05: Widefield vs laser scanning microscopy: adding the 3rd dimension
Homework 3, Due 10/20/10
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Oct
18
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Lecture 06: Practical considerations in a confocal microscope
** By 1pm email TAs the paper you will review for project 1
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Oct
20
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Lecture 07: Techniques for enhancing intrinsic contrast
** TAs will approve your paper selection or request a new one
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Oct
22
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Lecture
08: Resolution, contrast, and detection
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Nov
01
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Lecture
09: Multiple labeling and multi-spectral imaging
** Draft of project 1 paper due at the beginning of class! Exchange with peers to review.
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Nov
03
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Lecture
10: FRET
**Comments on your peer's paper due at beginning of class!
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Nov
05
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Lecture
11: Quantitative Fluorescence Microscopy
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Nov
08
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Lecture
12: Fast fluorescence and lightsheet microscopy
**Final revised project 1 paper due at beginning of class!
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Nov
10
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Lecture
13: Electron microscopy and tomography
(Grant Jensen)
**By 1pm email us the paper you will review for project 2.
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Eric Betzig's seminar on super-resolution microscopy
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Nov
17
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Lecture
14: MRI and MR microscopy (Russ Jacobs)
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Nov
19
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Lecture
15: Emerging imaging techniques I (NSOM, SIM, AFM, OPT, OCT)
** Draft of project 2 paper due at the beginning of class. Exchange for peer review.
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Nov
22
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Lecture
16: Emerging imaging techniques II (STED, 4pi, PALM/STORM)
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Nov
24
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**Complete peer review
and exchange comments.
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Dec
03
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**Final, revised
project 2 due by 1pm!
Please turn in to Danielle (Brown OR Bower)'s mailbox in the BI lobby:
1) your first draft of paper #2,
2) the peer comments you received, and
3) your final draft,
all paper-clipped together.
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Reading
Projects
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Read and study
a published paper in a mainstream journal. Write
a short report concerning this paper. The report should be ~5 pages
long. Get
pre-approval of TA on paper/topic.
Peer Review Form
Grading Criteria
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Coursework
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Reading
6
Simple problem sets
Paper
reading project (2 per term)
No
midterm or final exam
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Grading
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Grades
based on the Homework and the Projects.
Homework,
Project 1, Project 2 are given equal weight.
Late
homework assignments are not accepted.
Score
can be dropped with medical or Dean's excuse.
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Auditors
and non-traditional students
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Auditors
are welcome to take the Course, but are required to do the homework
problem sets and reading.
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Links
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Bi177
& Bi227 website
Microscope
Primer
Microscopy
from the Very Beginning (Zeiss)
Olympus
Microscopy Primer
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