Bi 177: Principles of Modern Microscopy
California Institute of Technology - Fall 2008
John Choi and Willy Supatto
TA: Judith Su
 

 

Hours
 
 
Mon and Wed 1:00-2:30 pm
Broad 100 Lecture Hall
 

 

Lecture schedule (updated 10/12)
 
Sept 29
 
Lecture 01: Basic optics (JC)
>download homework (due Oct 01)
 
Oct 01
 
Lecture 02: Optics of real microscopes (JC)
>download homework (due Oct 13)
note: Imaris software presentation on Oct 03 (2-3pm in Broad 100)
 
Oct 06
 
Science Writing Workshop with Steven Youra (Project 1)
 
Oct 08
 
Science Writing Workshop with TA (Project 1)
 
Oct 13
 
Lecture 03: Widefield light microscopy (JC)
>download homework (due Oct 22)
 
Oct 15
 
Lecture 04: Contrast techniques (JC)
 
Oct 20
 
Lecture 05: Fluorescent microscopy (JC)
 
Oct 22
 
Lecture 06: Electron Microscopy and Tomography (Grant Jensen)
>download homework (due Oct 31)
 
Oct 27
 
Lecture 07: Confocal microscopy I (JC)
 
Oct 29
 
Lecture 08: Confocal microscopy II (JC)
>download the reading
 
Nov 03
 
Lecture 09: Multiphoton microscopy I (2PEF) (WS)
 
Nov 05
 
Lecture 10: Multiphoton microscopy II (SHG, THG, CARS) (WS)
 
Nov 10
 
Science Writing Workshop with TA (Project 2)
 
Nov 12
 
Lecture 11: MRI and MR microscopy (Russ Jacobs)
 
Nov 17
 
Lecture 12: Motion and fast imaging (WS)
 
Nov 19
 
Lecture 13: Labeling and multi-spectral imaging (WS)
 
Nov 24
 
Lecture 14: Quantitative fluorescence microscopy (FLIM, FRET, FRAP, FCS) (WS)
 
Nov 26
 
Lecture 15: Digital imaging I: image formation, visualization and processing (WS)
 
Dec 01
 
Lecture 16: Digital imaging II: 3D-reconstruction, image restoration and analysis (WS)
 
Dec 03
 
Lecture 17: Fluorescence nanoscopy (NSOM, TIRF, 4Pi, STED, nonlinear SIM, STORM-PALM-FPALM) (WS)
 

 

Reading Projects
 
 
Read and study a published paper in a mainstream journal. Write a short report concerning this paper. The report should be ~5 pages long. Get pre-approval of TA on paper/topic.
>download the Reading Project Grading Criteria
 

 

Assignment Schedule (updated 10/12)
 
 
Reading Project 1:
 
Oct 06
 
Look for journal article (due Oct 13)
 
Oct 13
 
Write draft (due Oct 20*)
 
Oct 20
 
Do peer-review (due Oct 27*)
>download the Peer-Review Comment Sheet
 
Oct 27
 
Write final version of paper (due Oct 31)
 
 
Reading Project 2:
 
Nov 10
 
Look for journal article (due Nov 17)
 
Nov 17
 
Write draft (due Nov 24*)
 
Nov 24
 
Do peer-review (due Dec 01*)
 
Dec 01
 
Write final version of paper (due Dec 05**)
 
 
*due at beginning of class
** in TA mailbox
 

 

Course Work
 
 
Reading
Simple problem sets
Paper reading project (2 per term)
No mid-term of final exam
 

 

Grading
 
 
Grades based on the Homework and the Projects.
Homework, Project 1, Project 2 are given equal weight.
Late homework assignements are not accepted.
Score can be dropped with medical or Dean's excuse.
 

 

Auditors and non-traditional students
 
 
Auditors are welcome to take the Course, but are required to do the homework problem sets and reading.
 

 

Links
 
 
Bi177 & Bi227 website
Microscope Primer
Microscopy from the Very Beginning (Zeiss)
Olympus Microscopy Primer